TNF-α启动子5个位点的基因多态性与山东省汉族妇女宫颈癌遗传易感性的相关性  

Association between genetic polymorphisms of 5 genetic loci of TNF-α promoter and genetic susceptibility to cervical cancer

作　　者：赵庆庆 尹格平[2] 

机构地区：[1]锦州医科大学济南军区总医院培养基地妇科,山东济南250031 [2]济南军区总医院妇科,山东济南250031

出　　处：《山东大学学报（医学版）》2018年第2期28-33,共6页Journal of Shandong University:Health Sciences

基　　金：济南军区卫生部面上项目(2013ZX05)

摘　　要：目的探讨肿瘤坏死因子-α(TNF-α)启动子基因5个单核苷酸多态性(SNP)位点(rs361525、rs1800629、rs1800750、rs1799964、rs673)的基因型、等位基因频率与山东省汉族妇女宫颈癌发病易感性的相关性。方法采用Taq M an探针法对选取的TNF-α启动子5个SNP位点进行基因分型,分析比较宫颈癌组452例(其中原位癌组104例和浸润癌组348例)和对照组494例的基因类型与基因频率,并对两组进行流行病学调查与人乳头瘤病毒(HPV)亚型分析。结果与对照组比较,宫颈癌组rs361525(CT)、rs1800629(AG)和rs17999645(AG)基因型增加宫颈癌遗传风险倍数分别为3.040(95%CI:1.761~5.248,P=0.001)、2.580(95%CI:1.867~3.564,P=0.001)、3.162(95%CI:2.332~4.187,P=0.001)。rs1800750、rs673基因型频率在宫颈癌组及对照组之间差异无统计学意义(P=0.929,0.670)。5个SNP位点基因分型频率在浸润癌组与原位癌组间差异无统计学意义(P=0.124,0.455,0.437,0.809,0.868)。宫颈癌组HPV感染率(91.4%)高于对照组(10.3%)(P=0.001),浸润癌组(95.4%)HPV感染率高于原位癌组(77.9%)(P=0.001)。结论 TNF-α启动子rs361525、rs1800629、rs17999645单核苷酸基因多态性可能是山东省汉族妇女宫颈癌的遗传危险因素,rs1800750、rs673位点与山东省汉族妇女宫颈癌发生无明显相关性。Objective To investigate the correlation between the genotype and allele frequencies of 5 single nucleotide polymorphisms（ SNPs） in the promoter region of tumor necrosis factor-α（ TNF-α） gene（ rs361525,rs1800629,rs1800750,rs1799964 and rs673） and the susceptibility of cervical cancer in Han women in Shandong Province.Methods The 5 SNPs of TNF-α were genotyped by Taq Man probe and compared with 452 cases of cervical cancer（ 104 cases of carcinoma in situ and 348 cases of invasive carcinoma） and 494 controls in gene type and frequency. The epidemiological survey and HPV subtype analysis were conducted in the two groups. Results Compared with the controlgroup,the genetic risk of cervical cancer with rs361525（ CT）,rs1800629（ AG） and rs17999645（ AG） genotypes were 3.040（ 95%CI： 1.761-5.248,P = 0.001）,2.580（ 95%CI： 1.867-3.564,P = 0.001）,and 3.162（ 95%CI： 2.332-4.187,P = 0. 001）. The frequency of rs1800750 and rs673 genotypes were not significantly different between cervical cancer group and control group（ P = 0.929,0.670）. The frequency of genotype of the 5 SNP loci were not statistically different between the invasive cancer group and carcinoma in situ group（ P = 0.124,0.455,0.437,0.809,0.868）. The HPV infection rate in the cervical cancer group was 91.4%,which was higher than that in the control group（ 10.3%,P = 0.001）. The HPV infection rate in the in situ carcinoma was lower than that in invasive carcinoma（ 77. 9% vs95.4%,P = 0.001）. Conclusion The genetic polymorphisms of rs361525,rs1800629 and rs17999645 in TNF-α may be genetic risk factors for cervical cancer in Han women in Shandong Province,while there is no significant correlation between rs1800750 and rs673 loci and cervical cancer.

关 键 词：宫颈肿瘤 基因多态性 肿瘤坏死因子-Α 遗传易感性 山东省 汉族 

分 类 号：R711.74[医药卫生—妇产科学]