SD大鼠心血管系统的工频磁场暴露实验研究  

Exposure Experiment Research of the Power Frenquency Magnetic Field on the Cardiovascular System in SD Rats

作　　者：刘兴发[1,2] 唐雨萌 海景雯 何巍 陈琛 张业茂 万保权[1,2] 曲敏 唐利军[3,4] 

机构地区：[1]中国电力科学研究院,武汉430074 [2]电网环境保护国家重点实验室,武汉430074 [3]湖北省疾病预防控制中心(湖北省预防医学科学院),武汉430079 [4]应用毒理湖北省重点实验室(湖北省预防医学科学院),武汉430079 [5]华中科技大学同济医学院,武汉430030 [6]国网青海省电力公司电力科学研究院,西宁810008 [7]国网甘肃省电力公司电力科学研究院,兰州730050

出　　处：《高电压技术》2018年第2期599-609,共11页High Voltage Engineering

基　　金：国家电网公司科技项目(GY71–16–008);湖北省科技支撑计划(2014BEC065);国家自然科学基金(51607163)~~

摘　　要：为探讨长时间低磁感应强度的工频磁场暴露对SD大鼠心血管系统可能的影响,将128只SD大鼠随机均分为4组,其中3组设为暴露组,每天暴露于专门的工频磁场暴露系统中20 h时间,共持续24周时间,磁感应强度分别为30μT、100μT和500μT;另外1组设为对照组,除不进行工频磁场暴露之外,其他环境条件均与暴露组的相同;在暴露期间,每隔4周时间测量血压,24周时间后进行超声心动图、心导管及心电图测试;记录心脏质量和体质量,进行苏木精–伊红染色和实时定量荧光PCR（polymerase chain reaction）。研究结果表明：暴露组与对照组相比,磁感应强度为30μT、100μT和500μT的工频磁场暴露对SD大鼠的血压、脉率、心率和心律均无影响;超声心动图和心导管检查结果表明暴露组与对照组的SD大鼠心脏形态和血流动力学均无显著差异;组织病理学检查结果表明磁感应强度为30μT、100μT和500μT的工频磁场暴露对SD大鼠心脏结构均无影响;磁感应强度为30μT、100μT和500μT的工频磁场暴露组与对照组的SD大鼠心肌肥厚相关基因的表达均无显著性差异。总之,在磁感应强度为30μT、100μT和500μT的工频磁场中暴露24周时间,对SD大鼠心血管系统均没有明显的影响。In order to explore the possible effect of power frequency magnetic field exposure of long-term low magnetic induction intensity on the cardiovascular system in Sprague-Dawley（SD） rats, 128 SD rats were randomly divided into 4 groups. 3 groups of SD rats were exposed to the power frequency magnetic field with 30 μT, 100 μT and 500 μT for 24 weeks in specialized magnetic field exposure system, 20 h per day. Except for not being exposed to the frequency magnetic field, the control group had the same environmental conditions. During the exposure, blood pressure was measured every 4 weeks. After 24 weeks, echocardiography, cardiac catheterization and electrocardiography were performed. Heart and body weight were recorded, and haematoxylin-eosin staining and real-time PCR（Polymerase Chain Reaction） were conducted. The results of the experiments showed that compared with the control group, exposure to 30 μT, 100 μT and 500 μT magnetic field did not exert any effects on blood pressure, pulse rate, heart rate or cardiac rhythm. Echocardiography and cardiac catheterization showed that there were no significant differences in the cardiac morphology or haemodynamics. In addition, histopathological examination showed that exposure to 30 μT, 100 μT and 500 μT magnetic field had no effects on the structure of the heart. Finally, expression of the cardiac hypertrophy-related genes did not show any significant differences between the 30 μT, 100 μT and 500 μT magnetic field exposure groups and the control group. In SD rats, exposure to 30 μT, 100 μT and 500 μT power frequency magnetic field for 24 weeks did not show any obvious effects on the cardiovascular system.

关 键 词：工频磁场 暴露 SD大鼠 心血管系统 超声心动图 心导管 组织病理学 心肌肥厚相关基因 

分 类 号：R818.03[医药卫生—放射医学]