Genetic Variability of the Mitochondrial DNA in Honeybees （Apis mellifera L.） from Benin  

作　　者：Aude Kelomey Armand Paraiso Haziz Sina Helene Legout Adolphe Adjanohoun Lionel Gamery Lamine Baba-Moussa 

机构地区：[1]Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Sciences and Techniques, University of Abomey-Calavi,05 P.O. Box 1604, Cotonou, Benin [2]Laboratory of Bees Pathology, Parasitology and Plant Protection (LAPPAB), University of Parakou, P.O. Box 123, Parakou,Benin [3]Laboratory of Evolution, Genomes and Speciation (EGCE), French National Centre for Scientific Research (CNRS),Gif-sur- Yvette 91190, France [4]Centre de Recherche Agricole Sud, Institut National des Recherche Agricoles du Benin, Attogon, P.O. Box 884, Cotonou, Benin

出　　处：《Journal of Agricultural Science and Technology(A)》2017年第8期557-566,共10页农业科学与技术（A）

摘　　要：The aim of this study was to evaluate the genetic variability in bees Apis mellifera from Benin by using mitochondrial DNA （mtDNA） as a molecular marker in their cytochrome c oxidase subunit I and II （COI-COI1） intergenic region. A total of 304 bee colonies were sampled in 27 municipalities of the cashew growing area of Benin. These samples were analyzed by the cleaved amplified polymorphisms technique for determining the haplotypes of subspecies present in the sampled population. Eight PCR-RFLP profiles of African lineage A were then identified in the 304 samples of bees investigated. Forty-nine percent （49%） of the samples showed the profile of haplotype A1 （subspecies adansonii of Zambia）, 40% of haplotype A4 （subspecies scutellata of South Africa） and 3% of haplotype A 19 （subspecies adansonii of Guinea）. Five other haplotypes of the African branch （A） that had been described in a previous study were also identified： new 1 （2%）, new 2 （2%）, new 3 （1%）, new 4 （2%） and new 5 （1%）. This study showed that A. rnellifera from Benin belonged only to lineage A with the predominance of haplotypes AI and A4. This study will contribute to the development of coherent policies for conservation of local bees in Benin.

关 键 词：Apis mellifera adansonii mitochondrial DNA cytochrome c oxidase subunit Ⅰ and Ⅱ African lineage Benin. 

分 类 号：Q523[生物学—生物化学] S894[农业科学—特种经济动物饲养]