Effects of Compound Zhebei Granule(复方浙贝颗粒) Combined with Doxorubicin on Expression of Specific Surface Antigens in Mice with Transplanted KG-1a Cells  

作　　者：ZHANG Yu HOU li CHEN Xin-yi 

机构地区：[1]Department of Integrated Chinese and Western Medicine,Tianjin Medical University Cancer Institute and Hospital,National Clinical Research Center for Cancer Tianjing 300060,China [2]Key Laboratory of Cancer Prevention and Therapy,Tianjin 300060, China [3]Tianjin Clinical Research Center for Cancer, Tianjin 300060, China [4]Department of Hematology,Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine, Beijinq 100700, China

出　　处：《Chinese Journal of Integrative Medicine》2018年第3期213-217,共5页中国结合医学杂志（英文版）

摘　　要：Objective：To investigate the effects of Compound Zhebei Granule（复方浙贝颗粒,CZG）combined with doxorubicin hydrochloride（adriamycin,ADM）on specific surface antigens in mice with KG-1a transplanted cells.Methods：A subcutaneous tumor xenograft model was established by injection of the acute myeloid leukemia cell line KG-1a into the axillary flanks of BALB/c-nude mice.Twenty-four tumor bearing mice were divided into 4groups according to a random number table,including normal saline control group,ADM group,high-dose CZG group,and mid-dose CZG group,with 6 mice in each group.Drug administration occurred on the 14th day after cell inoculation,and normal saline control group mice were gavaged with normal saline at 0.2 m L/10 g every other day.ADM group mice were intraperitoneally injected with ADM 1 mg/kg[conversion of adults,40 mg/（m^2·d）]every other day.High-and mid-dose CZG groups mice were gavaged with CZG at the dose of 8 and 4 g/kg respectively every other day and intraperitoneally injected with ADM（1 mg/kg）every other day.The administration period lasted for10 days.The tumor xenografts were made into mononuclear cell suspensions after dissection,and the expressions of specific surface antigens,including CD34~＋CD38^-,CD34~＋CD38^-CD123~＋,CD34~＋CD38^-CD96~＋and CD34~＋CD38^-CD33~＋,in KG-1a cell line tumor xenografts were detected by flow cytometry.Results：Compared with the control and ADM groups,expression of CD34~＋CD38^-in the two CZG groups was significantly lower（P〈0.05）.Compared with the control group,expression of CD34~＋CD38^-CD123~＋in the two CZG groups decreased（P〈0.05）.The high-dose CZG group showed more obvious outcomes compared with the ADM group（P〈0.05）.Compared with the control and ADM groups,the expression of CD34~＋CD38^-CD96~＋and CD34~＋CD38^-CD33~＋in the two CZG groups decreased（P〈0.05）.Conclusions：CZG combined with doxorubicin could reduce the expression of CD34~＋CD38^-,CD34~＋CD38^-CD123~＋,CD34~＋CD38^-CD96~＋and Objective：To investigate the effects of Compound Zhebei Granule（复方浙贝颗粒,CZG）combined with doxorubicin hydrochloride（adriamycin,ADM）on specific surface antigens in mice with KG-1a transplanted cells.Methods：A subcutaneous tumor xenograft model was established by injection of the acute myeloid leukemia cell line KG-1a into the axillary flanks of BALB/c-nude mice.Twenty-four tumor bearing mice were divided into 4groups according to a random number table,including normal saline control group,ADM group,high-dose CZG group,and mid-dose CZG group,with 6 mice in each group.Drug administration occurred on the 14th day after cell inoculation,and normal saline control group mice were gavaged with normal saline at 0.2 m L/10 g every other day.ADM group mice were intraperitoneally injected with ADM 1 mg/kg[conversion of adults,40 mg/（m^2·d）]every other day.High-and mid-dose CZG groups mice were gavaged with CZG at the dose of 8 and 4 g/kg respectively every other day and intraperitoneally injected with ADM（1 mg/kg）every other day.The administration period lasted for10 days.The tumor xenografts were made into mononuclear cell suspensions after dissection,and the expressions of specific surface antigens,including CD34~＋CD38^-,CD34~＋CD38^-CD123~＋,CD34~＋CD38^-CD96~＋and CD34~＋CD38^-CD33~＋,in KG-1a cell line tumor xenografts were detected by flow cytometry.Results：Compared with the control and ADM groups,expression of CD34~＋CD38^-in the two CZG groups was significantly lower（P〈0.05）.Compared with the control group,expression of CD34~＋CD38^-CD123~＋in the two CZG groups decreased（P〈0.05）.The high-dose CZG group showed more obvious outcomes compared with the ADM group（P〈0.05）.Compared with the control and ADM groups,the expression of CD34~＋CD38^-CD96~＋and CD34~＋CD38^-CD33~＋in the two CZG groups decreased（P〈0.05）.Conclusions：CZG combined with doxorubicin could reduce the expression of CD34~＋CD38^-,CD34~＋CD38^-CD123~＋,CD34~＋CD38^-CD96~＋and

关 键 词：acute myeloid leukemia Chinese medicine doxorubicin adriamycin 

分 类 号：R285.5[医药卫生—中药学]