人羊膜间充质干细胞不同移植方式治疗大鼠脊髓损伤疗效比较  

Varied therapeutic effects of Human Amniotic Mesenchymal Stem Cell Transplantation with Different Transplantation Methods for Spinal Cord Injury Therapy in Rats

作　　者：马晶晶 廖旭勇 赵玉洁 喻皇飞 

机构地区：[1]遵义医学院第三附属医院检验科,遵义563000 [2]遵义医学院第一附属医院检验科,遵义563000 [3]遵义医学院第三附属医院肿瘤科,遵义563000

出　　处：《实用医学杂志》2018年第4期562-566,571,共6页The Journal of Practical Medicine

基　　金：贵州省联合基金项目(编号:黔科合J字LKZ(2010)19号)

摘　　要：目的比较原位移植和尾静脉注射途径移植人羊膜间充质干细胞(h AMSCs)对脊髓损伤大鼠组织学修复及神经功能的影响。方法建立大鼠脊髓损伤模型,1周后通过脊髓原位或尾静脉移植DAPI标记的h AMSCs细胞,BBB评分评估大鼠后肢运动功能,组织学观察受损脊髓组织Neu N、GFAP、CD68的表达和髓鞘的形态改变。结果 h AMSCs移植后,原位注射和尾静脉移植组大鼠后肢功能BBB评分逐周增加,至第6周时分别是6.71±0.70和6.93±1.28,均高于PBS对照组(均P<0.01),但两组间比较无统计学意义。组织学结果显示,PBS对照组损伤脊髓组织中空洞、囊腔形成明显;Neu N在h AMSCs移植后大鼠脊髓中的表达高于对照组;GFAP、CD68阳性表达细胞则在相应对照组脊髓残端聚集分布,较h AM-SCs移植组明显增多。PBS对照组大鼠病损脊髓髓鞘板层松懈,空泡化严重,而无论h AMSCs原位移植或尾静脉移植组大鼠脊髓组织中髓鞘的毁损改变均明显轻于对照组。结论 h AMSCs静脉移植能够促进脊髓损伤大鼠的神经功能重建和组织学修复,具有与原位移植相似的治疗效果。Objective To compare the therapeutic effects of orthotopic injection and tail vein injection of human amniotic mesenchymal stem cells （hAMSCs） on histological restoration and neurological functions of rats with spinal cord injury. Methods Transected spinal cord injury model in rats was established by transplanting DAPI prelabelled hAMSCs one week after injury. BBB scores were used to evaluate the hindlimb movement of rats. The histological patterns.and morphology of medullary sheath of spinal cord were observed. Results BBB scores in the orthotopic injection group and tail vein injection group were increased gradually from one to six week after hAMSCs transplantation and reached 6.5 ±0.5 and 7.12 ± 1.61 respectively 6 weeks after cell transplantation, higher than that of the control group （both P 〈 0.01）. However, there was no statistical significance between the two groups. Histological results indicated that the repair of injured tissue in the orthotopic injection group and tail vein injection group were both better than that in the control group, and there were more vesica and loosened layers forming in the injured spinal cord of rats in the PBS control group as compared with the orthotopic and tail vein transplantation group. Conclusion hAMSCs transplantation through tail vein injection could promote histological restoration and neurological regeneration of rats with spinal cord injury, which has the similar therapeutic effects with hAMSCs orthotopic transplantation.

关 键 词：人羊膜间充质干细胞 脊髓损伤 移植 BBB评分 

分 类 号：R651.2[医药卫生—外科学]