不同产地黄连中重金属的含量测定及不同用药方式下黄连重金属的风险评估  

Determination of heavy metals in Coptidis Rhizoma of different habitats and risk assessment of heavy metals under different ways of administration

作　　者：周利[1,2] 杨健 詹志来[1,3] 舒少华 王升[1,3] 邓爱平[1,2] 马忠华[5] 郭兰萍 

机构地区：[1]中国中医科学院中药资源中心,道地药材国家重点实验室培育基地,北京100700 [2]广东药科大学中药学院,广东广州510006 [3]中国中医科学院中药资源中心无限极中草药品质研究联合实验室,北京100700 [4]华中农业大学植物科学技术学院,湖北武汉430070 [5]无限极(中国)有限公司,广东广州510180

出　　处：《药学学报》2018年第3期432-438,共7页Acta Pharmaceutica Sinica

基　　金：国家药典委员会药品医疗器械审评审批制度改革项目(ZG2016-1); 中国中医科学院重点领域(ZZ10-27); 国家重点研发计划(2017YFC1700701)

摘　　要：为了解黄连中重金属整体残留情况,并对黄连中重金属进行风险评估,本研究利用微波消解-ICP-MS法测定了不同产地黄连中重金属含量;利用煎煮法及人工胃肠消化模型,分别模拟黄连煎煮入药及原粉入药的服用方式,测定重金属在两种入药方式下的转移率,并利用靶标危害系数法(THQ)对黄连的重金属风险进行评估。结果显示,在ISO国际标准下,17批黄连样品中重金属Cu、Pb、As、Cd、Hg含量的超标率分别为0、12%、0、0、0。在水煎液和人工胃肠提取液中,重金属Cu、Pb、As、Cd、Hg的转移率分别为3.63%、1.69%、37.17%、20.86%、0和59.15%、29.98%、67.55%、104.59%、0。通过风险评估模型计算成人和小孩在两种用药方式下的THQ分别为0.001 0、0.005 3和0.000 7、0.003 6,黄连重金属限量理论值(MRL)均大于本研究样品中重金属含量。提示本研究中黄连重金属含量处于安全范围,不会对人体产生明显危害;黄连中重金属超标问题有可能为标准过严造成。本研究考察了不同产地黄连中重金属残留情况和黄连不同入药方法下重金属的溶出情况,采用国际通用的健康风险评估模型对黄连中重金属风险进行评估,为黄连重金属限量标准的制定奠定了基础。Inductively coupled plasma mass spectrometry（ICP-MS） was applied to this study to detect heavy metal contents in Coptidis Rhizoma from different habitats, for a comprehensive understanding of heavy metal residues in Coptidis Rhizoma. Decocting method and artificial gastrointestinal digestion model were used to determine transfer rates of heavy metals in assessment of health risk of heavy metals using the target hazard quotient（THQ） developed by the US EPA（1989）. The results showed that excess rates of Cu, Pb, As, Cd and Hg of 17 batches of Coptidis Rhizoma were 0, 12%, 0, 0 and 0, respectively, under the ISO international standard of Chinese medicine-Chinese herbal medicine heavy metal. The transfer rates of Cu, Pb, As, Cd and Hg were 3.63%, 1.69%, 37.17%, 20.86% and 0 in decoction solution, respectively, and 59.15%, 29.98%, 67.55%, 104.59% and 0 in artificial gastrointestinal solution, respectively. The values of THQ under the two ways of administration in adults and children were 0.001 0, 0.005 3 and 0.000 7, 0.003 6, respectively, and the maximum residue limits（MRL） of heavy metals in Coptidis Rhizoma were higher than the contents of samples in this study. The research showed that the contents of heavy metals in Coptidis Rhizoma were in the safe ranges with no obvious risks to human body, indicating that the excessive of heavy metals in Coptidis Rhizoma might be attributed to the unduly strict standard. The contents of heavy metals in Coptidis Rhizoma of different habitats was estimated for health risks using international risk assessment model, which provides a reference for establishment of heavy metal standards in Coptidis Rhizoma.

关 键 词：黄连 重金属 入药方式 风险评估 

分 类 号：R931[医药卫生—生药学]