不同健康程度和抗生素氟苯尼考干预下斑石鲷肠道菌群的结构差异  

Variance analysis of bacterial community in the intestine of cultured spotted knifejaw(Oplegnathus punctatus) at different healthy levels and intervened with florfenicol

作　　者：许燕 王印庚[2] 张正[2] 姜燕[2] 廖梅杰[2] 李彬[2] 王凯[1,2] 李文生 

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]中国水产科学研究院黄海水产研究所青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室,山东青岛266071 [3]莱州明波水产有限公司,山东烟台261418

出　　处：《水产学报》2018年第3期388-398,共11页Journal of Fisheries of China

基　　金：国家科技支撑计划(2012BAD17B03);山东省自主创新成果转化专项(2013ZHZX2A0801)~~

摘　　要：为了研究不同健康程度和抗生素氟苯尼考干预下斑石鲷肠道菌群结构的差异及其与养殖环境中菌群结构的相关性,采用Illumina Hi Seq PE250高通量测序的方法对健康、亚健康、典型黑身病和口服氟苯尼考条件下的斑石鲷肠道、养殖水体和颗粒饵料中的细菌多样性及群落结构进行了分析比较。结果显示,养殖水体中细菌多样性高于肠道和颗粒饵料。不同健康程度及氟苯尼考干预下斑石鲷肠道中细菌均以变形菌门、厚壁菌门和软壁菌门为主,且对应的操作分类单元(OTU)占样品全部OTU的比例均达到85%以上。黑身病的发生可影响斑石鲷肠道中丰度最高的前20种优势细菌种类的排名次序,其中变形菌门中的弧菌属的相对丰度显著增加,且随着弧菌属丰度的增加,斑石鲷的黑身病症状也逐渐加重。饵料中添加氟苯尼考投喂斑石鲷能使患病鱼肠道弧菌属的丰度从60.33%下降到1.29%,较大程度改变了肠道的菌群结构,并证实氟苯尼考有效防治黑身病。其次,养殖水体和颗粒饵料对斑石鲷肠道菌群也有一定影响,且养殖水体的影响高于颗粒饵料。本研究首次报道了斑石鲷肠道菌群结构,其研究结果为今后斑石鲷的健康养殖、疾病防控及其微生态学研究提供了参考依据和技术支撑。In order to evaluate the changes of the bacterial communities in the intestine of spotted knifejaw（Oplegnathus punctatus） at different healthy levels and intervened with florfenicol, and to find out the relationship with environmental factors, the Illumina Hi Seq PE250 high-throughput technique was used to analyze the bacterial community diversity and structure of the fish intestine for healthy, sub-healthy, typical dark body disease（DBD）infected and oral florfenicol intervened individuals, as well as the culture water and pellet feed. The results showed that bacterial diversity in the culture water was higher than that of intestine and pellet feed. The dominant bacterial communities in intestine were Proteobacteria, Firmicutes and Tenericutes, at different health levels and with florfenicol intervention, and the proportions of corresponding operational taxonomic unit（OTU） number to all the OTU in the samples were above 85%. It could affect the ranking order of the top 20 dominant bacterial species in intestine when the DBD occurred, with the relative abundance of Vibrio in Proteobacteria increasing significantly. In addition, the DBD symptoms aggravated gradually, with the increase of Vibrio abundance. Feeding with florfenicol could reduce the Vibrio abundance from 60.33% to 1.29% in the intestine of diseased fish, and also change its structure of bacterial community to a greater degree, which proved that florfenicol was effective in treating DBD.It was also found that the culture water and pellet feed had a certain influence on intestine bacterial community,and the effect of the culture water was higher than the pellet feed. The present results would provide a reference basis and technical support for the health culture, disease control and microecological study in spotted knifejaw.

关 键 词：斑石鲷 黑身病 氟苯尼考 肠道菌群 高通量测序 操作分类单元(OTU) 

分 类 号：S917.1[农业科学—水产科学]