超高效液相色谱-串联质谱法同时检测动物源性食品中残留的35种β-受体激动剂和11种β-受体阻断剂  

Simultaneous determination of 35β-agonist and 11β-blocker residues in animal-origin foods by ultra high performance liquid chromatography-tandem mass spectrometry

作　　者：史娜 郝杰 姜洁 邵瑞婷 张丽华 孙晓冬 王丽 

机构地区：[1]北京市食品安全监控和风险评估中心,北京100041

出　　处：《分析仪器》2018年第1期20-31,共12页Analytical Instrumentation

基　　金：北京市科技计划课题;Z161100000616007

摘　　要：针对动物肌肉、肝脏等动物源性食品建立了一种超高效液相色谱-串联质谱法同时检测动物源性食品中残留的35种β-受体激动剂和11种β-受体阻断剂残留量的方法。通过优化萃取溶剂、净化条件、样品基质效应的消除、色谱-质谱条件等参数,提高检测方法的灵敏度和适用性。并且对定量检测方法阿德灵敏度、准确度、精密度等指标进行了验证。结果表明:46种化合物在0.05μg/kg^10μg/kg的空白添加浓度范围内呈良好的线性关系,平均回收率为62.31~110.52%,相对标准偏差为1.03~13.33%。该方法快速、灵敏、准确,稳定,适用于食品安全突发事件的快速应急处置工作。To improve the sensitivity and applicability of the method,several conditions and parameters were optimized,including the extraction solvent,purification method,removal of matrix effect and analysis conditions of UPLC-MS/MS.Meanwhile,the sensitivity,accuracy and precision of the quantitative method were validated.The result showed that all the 46 kinds of compounds ranged 0.05-10μg/kg with good linear relationship in sample matrixes.Ther average recoveries of the method were 62.31% -110.52% with RSD of 1.03% -13.33% .The method is rapid,sensitive,accurate,stable,and suitable for rapid response of food safety emergency.

关 键 词：超高效液相色谱-串联质谱 同时检测 β-受体激动剂 Β-受体阻断剂 动物源性食品 

分 类 号：O657.63[理学—分析化学] TS207.53[理学—化学]