斑马鱼中Wnt信号通过调控侧线基板形成影响侧线发育机制  

The Wnt signaling affects development of the lateral line by regulating the specification of lateral line placode in the zebrafish

作　　者：葛玲玲 宋佳坤[1,2,3,4] 范纯新[1,2,3,4] 

机构地区：[1]上海海洋大学海洋生物系统与神经科学研究所,上海201306 [2]上海海洋大学水产科学国家级实验教学示范中心,上海201306 [3]上海海洋大学海洋生物科学国际联合研究中心中国科学技术部,上海201306 [4]上海海洋大学水产种质资源发掘与利用教育部重点实验室,上海201306

出　　处：《上海海洋大学学报》2018年第1期16-23,共8页Journal of Shanghai Ocean University

基　　金：上海海洋大学国际海洋研究中心海洋生物系统和神经科学研究所(A1-0203-00-4007);高峰团队重点实验室宋佳坤团队经费(A1-2018-14-0005-2);上海高校水产学一流学科建设项目(A2-2019-14-001-4)

摘　　要：鱼类侧线系统源自胚胎期的侧线基板,侧线基板的特化受到多种信号的综合作用。利用转基因斑马鱼hsp:wnt8a-egfp和hsp:dkk1-egfp,增强或抑制Wnt信号,通过YO-PRO-1染色和毛细胞计数,研究斑马鱼中Wnt信号对侧线系统发育的影响。热激诱导Dkk1过表达后,斑马鱼侧线系统的神经丘不能形成。碱性磷酸酶染色和整体原位杂交结果表明:Wnt信号是神经丘毛细胞前体细胞以及侧线基板标记基因Eya1和Six2b表达所必需的。综上结果:Wnt信号可能通过调控侧线基板形成影响斑马鱼的侧线发育。The lateral line system originates from the embryonic lateral line placode.The specification of lateral line placode requires the collaboration of a variety of signalings.In order to study the role of Wnt signaling in the development of the lateral line system in zebrafish,we modulated the Wnt signaling by heating shock of the transgenic zebrafish,hsp： Wnt8 a-egfp and hsp： dkk1-egfp,and evaluated the hair cell number by YO-PRO-1 staining.We found that hair cells in the zebrafish lateral line system cannot form normally after overexpression of Dkk1.Furthermore,we found Wnt signaling was also necessary for precursor cells formation using alkaline phosphatase staining and in situ hybridization of Eya1 and Six2 b.In summary,Wnt signaling affects the development of lateral line system through regulating lateral line placode formation.

关 键 词：斑马鱼 侧线基板 毛细胞 WNT信号 

分 类 号：S917[农业科学—水产科学]