海洋酸化对海水青鳉性别分化的影响  

Effects of ocean acidification on the sexual differentation of marine medaka Oryzias melastigma

作　　者：宋露露 王晓杰[1,2,3,4] 宋佳坤[1,2,3,4] 

机构地区：[1]上海海洋大学海洋生物系统与神经科学研究所,上海201306 [2]上海海洋大学水产科学国家级实验教学示范中心,上海201306 [3]上海海洋大学海洋生物科学国际联合研究中心中国科技部,上海201306 [4]上海海洋大学水产种质资源发掘与利用教育部重点实验室,上海201306

出　　处：《上海海洋大学学报》2018年第1期29-36,共8页Journal of Shanghai Ocean University

基　　金：国家自然科学基金(41306097);中国科学院海洋生态与环境科学重点实验室开放课题(KLMEES2013);上海海洋大学国际海洋研究中心海洋生物系统和神经科学研究所项目(A1-0203-00-4007);高峰团队重点实验室宋佳坤团队项目(A1-2018-14-0005-2)

摘　　要：以海水青鳉作为研究对象,研究海洋酸化对其性别分化的影响。将刚受精的受精卵置于对照组(CO_2浓度为480×10^(-6))和2个处理组(CO_2浓度分别为1 000×10^(-6)和2 000×10^(-6))的水体中,直到孵化出膜。将出膜后仔鱼饲养在对照组水体中至性成熟,然后通过形态学、组织学和遗传学方法,对每一尾实验鱼进行表现型和基因型的性别鉴定。经形态学判断,发现酸化处理组的雄雌比例显著高于对照组(P<0.01)。经形态学观察其第二性征和遗传学性别判断,对照组的青鳉从孵化到性成熟的个体中并未出现基因型和表现型性别不一致的个体,而在2个酸化处理组中,均有基因型为XX而表现型为雄性的个体,即性逆转雄性,性逆转率分别为38.4%和47.5%。In this study,the effect of ocean acidification on sex differentation of marine medaka was studied.Fertilized eggs were incubated at three concentrations of CO2（480 × 10-6,1 000 × 10-6 and 2 000 × 10-6）and after hatching,the fry were bred under control condition（480 × 10-6） until adulthood.Phenotypic sex was judged from secondary sexual characteristics.The ratio of male to female in two treatment groups was significantly higher than that in the control group（P 〈 0.01）.Genotypic sex was determined by the presence or absence of the DMY gene.In the control group,phenotypic sex completely agreed with genotypic sex.However,in two treatment groups,there are some phenotypical males but genotypic female,which is sexreversed XX male.The sex reversal rate was 38.4% and 47.5% at 1 000 × 10-6 and 2 000 × 10-6 concentration of CO2,respectively.

关 键 词：海洋酸化 性别分化 胚胎发育 雄雌比例 性逆转 

分 类 号：S917[农业科学—水产科学]