不同发育时期小鼠脑海马组织中GSK-3β和SUMO-1的表达研究  

Expression of GSK-3β and SUMO-1 in the Hippocampus of Mice at Different Developmental Stages

作　　者：李奉权 杨晨宇 

机构地区：[1]武汉大学基础医学院,湖北武汉430071 [2]湖北民族学院医学院,湖北恩施445000

出　　处：《湖北民族学院学报（医学版）》2018年第1期1-4,共4页Journal of Hubei Minzu University(Medical Edition)

基　　金：2016国家级和湖北省省级大学生创新创业训练计划项目(201610517015)

摘　　要：目的探讨糖原合酶激酶3β(GSK-3β)及小泛素样修饰蛋白-1(SUMO-1)在C57小鼠不同发育时期脑海马中的表达量及其活性的变化和意义。方法采用激光共聚焦技术检测SUMO-1过表达时GSK-3β在HEK 293细胞内的共定位及其表达情况,Western blot法检测和分析小鼠不同发育时期脑海马中GSK-3β和SUMO-1的表达量及GSK-3β活性变化情况。结果 SUMO-1过表达时GSK-3β与SUMO-1在HEK 293细胞中能共定位且GSK-3β表达增加;2周龄(P14)、1月龄(P30)、4月龄(P120)、8月龄(P240)小鼠脑海马中GSK-3β和SUMO-1表达量无明显变化,而12月龄(P360)和18月龄(P540)小鼠脑海马中GSK-3β和SUMO-1增加(P<0.05),GSK-3β第216位苏氨酸的磷酸化水平明显升高。结论 GSK-3β可能被SUMO-1化修饰,且GSK-3β在小鼠老年期其表达量增多,活性增加。Objective To explore the expression level and activity of the glycogen synthase ki- nase 3β （GSK-3β） and small ubiquitin-related modified proteins-1 （SUMO-1） in the hippo- campus of C57 mice＇s brain at different development stages.Method Laser Scanning Confocal Microscope was used to detect whether GSK-3β and SUMO-1 were co-localized when SU- MO-1 overexpressed in the HEK 293 cells.Western blot was used to test the expression level of GSK-3β and SUMO-! and the activity of GSK-3β in the hippocampus of mice＇s brain at dif- ferent development stages.Resells When SUMO-1 overexpressed GSK.--3β co-localizing with SUMO-1 in HEK 293 cells,the expression level of GSK-3β was also increased.There was no significant change of the expression level of GSK -3β and SUMO-1 in the hippocampus of mice aged 2 weeks （ P14）, 1 month （P30） ,4 months （P120） ,8 months （P240） ,while both GSK -313 and SUMO-1 expression in the hippocampus were increased in mice aged 12 months （P360） and 18 months （P540） （P〈O. 05）.The phosphorylation level of GSK -3β （Tyr216） was up-regulated.Cenclusien GSK-3β may be modified by SUMO-1. Furthermore , the expression and activity of GSK-3β was increased in the aged mice.

关 键 词：GSK-3Β SUMO-1 发育 表达 

分 类 号：R338[医药卫生—人体生理学]