ASC与Caspase-1在PBC患者外周血中的表达研究  

Study on expression of ASC and Caspase-1 in peripheral blood of patients with primary biliary cirrhosis

作　　者：樊佳 赵二川 叶震璇[2] 李红梅[2] 任智晶 张华[2] 

机构地区：[1]贵州医科大学临检教研室,贵阳550004 [2]贵州省人民医院检验科,贵阳550002

出　　处：《重庆医学》2018年第8期1044-1048,共5页Chongqing medicine

基　　金：贵州省科学技术厅项目(黔科合LH字[2014]7008号)

摘　　要：目的探讨ASC与Caspase-1在原发性胆汁性肝硬化(PBC)发病过程中的影响。方法采用实时荧光定量PCR、Western blot、焦磷酸测序及ELISA等方法分别检测30例PBC患者及健康对照者外周血单个核细胞(PBMC)中Caspase-1与ASC的mRNA和蛋白的相对表达情况,以及ASC启动子区甲基化状况和血浆中Caspase-1和IL-18的表达水平。结果 PBC组患者PBMC中Caspase-1、ASC mRNA和蛋白表达明显高于对照组(P<0.05)。ASC启动子区域Island 1(IS1)各位点甲基化率明显低于对照组(P<0.05),Island 2(IS2)各位点甲基化率小于背景值,未发生甲基化。PBC组血浆中Caspase-1与IL-18的水平明显高于对照组(P<0.05)。ASC mRNA与Caspase-1mRNA的表达呈明显正相关(P<0.05);ASC启动子区域IS1的第1、2、4和5位点的甲基化率与ASC mRNA的表达均呈负相关(P<0.05),第3、6位点甲基化率则与其表达无相关性(P>0.05);血浆中Caspase-1与IL-18水平呈明显正相关(P<0.05)。结论 ASC和Caspase-1参与了PBC的免疫炎性反应。Objective To explore the influence of apoptosis-associated speck-like protein containing a caspase recruitment do main（ASC） and Caspase-1 on the pathogenesis of primary biliary cirrhosis （PBC）. Methods The real-time PCR,Western blot,py- rophosphate sequencing and ELISA were adopted to respectively detect the relative expressions of mRNA and protein of peripheral blood mononuclear cells（PBMS） Caspase-1 and ASC as well as the methylation status of ASC promoter region and plasma Caspase 1 and IL-18 expression levels in 30 cases of PBC and healthy controls. Results The mRNA and protein expressions of PBMC Caspase-1 and ASC in the PBC group were significantly higher than those in the control group （P〈0.05）. The methylation rate of ASC promoter Islandl（ISI） was significantly lower than that of the control group（P〈0.05）, which of Island 2 （IS2） was smaller than the background value and had no methylation occurrence. The levels of plasma Caspase-1 and IL-18 in the PBC group were significantly higher than those in the control group （P〈0.05）. The ASC mRNA in the PBC group was significantly correlated with the Caspase-1 mRNA expression（P〈0.05）; the methylation rates at loci 1,2,4,5 of ASC promoter region CpG island were negatively correlated with ASC mRNA expression （P〈 0. 05）, and which at loci 3,6 had no correlation with their expressions （P〉0.05） ;plasma Caspase-1 and IL-18 levels showed the obviously positive correlation. Conclusion ASC and Caspase-1 are involved in the immune inflammatory response in PBC.

关 键 词：肝硬化 胆汁性 半胱氨酸蛋白酶类 ASC 焦磷酸测序 

分 类 号：R446.6[医药卫生—诊断学]