口蹄疫病毒感染中乳鼠epsilon干扰素mRNA转录水平的实时荧光定量PCR检测  

Detection of interferon epsilon mRNA transcription level of suckling mouse during exposure to foot-and-mouth disease virus infection by real time quantification PCR

作　　者：李世芳[1] 赵付荣[1] 邵军军[1] 常惠芸[1,2] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室国家口蹄疫参考实验室,甘肃兰州730046 [2]江苏省动物重要疫病与人兽共患病防控协同创新中心,江苏扬州225009

出　　处：《中国兽医科学》2018年第3期269-274,共6页Chinese Veterinary Science

基　　金：国家重点研发计划项目——牛羊重大动物疫病基因工程疫苗及防控研究(2017YFD0500902)

摘　　要：利用3～4日龄的乳鼠作为研究对象,在口蹄疫病毒（FMDV）MYA/98株攻毒后第0、5、10小时及濒临死亡期（第15小时）收集其肠道组织并提取RNA,用实时荧光定量PCR检测乳鼠IFN-ε表达量在感染前后及不同时间段的变化。结果显示,IFN-ε表达量在乳鼠感染FMDV前后以及不同时间段并没有发生明显的变化。本研究结果为进一步了解Ⅰ型干扰素的不同亚型及其在抗口蹄疫病毒感染中的作用提供了实验数据。The suckling mice aging from 3—4 days-old were employed and the intestinal tissue was collected at 0 h,5 h,10 h,and 15 h after the mice were challenged with foot-and-mouth disease virus（FMDV）MYA/98 strain.Real-time quantitative PCR was utilized to detect the IFN-ε mRNA transcription levels of suckling mouse before and after challenge.The results showed that IFN-ε expression would not alter between uninfected and acutely infected intestinal tissue or between the difference time-points postinfection.These results provided some experimental data for further understanding the subtypes of typeⅠinterferon and their role in anti-infection of FMDV infection.

关 键 词：干扰素 乳鼠 IFN-ε 荧光定量PCR 

分 类 号：S852.659.5[农业科学—基础兽医学]