骚扰阿蚊线粒体全基因组序列测定和分析  

Sequencing and analysis of the complete mitochondrial genome of Armigeres subalbatus(Diptera:Culicidae)

作　　者：李旭东[1] 陈斌[1] 

机构地区：[1]重庆师范大学昆虫与分子生物学研究所,媒介昆虫重庆市重点实验室,动物生物学重庆市重点实验室,重庆401331

出　　处：《昆虫学报》2018年第1期114-121,共8页Acta Entomologica Sinica

基　　金：“两江学者”计划专项经费(20136666);国家自然科学基金项目(31672363,31372265);国际原子能机构CRP项目(18268);国家科技基础性工作专项重点项目(2015FY210300);广西科技重大专项(桂科AA17129002)

摘　　要：【目的】测定和分析骚扰阿蚊Armigeres subalbatus线粒体全基因组序列,并在线粒体基因组水平探讨阿蚊属Armigeres在库蚊亚科(Culicinae)中的系统发育地位。【方法】经PCR扩增和序列测定,首次得到骚扰阿蚊线粒体基因组序列;对其核苷酸组成和结构特点进行分析;基于蛋白质编码基因核苷酸序列,采用最大似然法(ML)和贝叶斯法(BI)构建库蚊亚科8个种的系统发育关系。【结果】骚扰阿蚊线粒体基因组全长14 891 bp(Gen Bank登录号:KY978578),包含37个基因,其中含13个蛋白质编码基因、22个tRNA基因和2个rRNA基因,各基因位置、排列顺序与蚊科已知物种的一致;基因组碱基组成具有明显的偏好性,全基因组AT-skew为正值,GC-skew为负值;13个蛋白质编码基因的起始密码子除COⅠ使用TCG外,其余均为ATN,终止密码子除COⅡ使用不完全的T外,其余均为TAA;22个tRNA基因中除tRNA^(Ser(AGN))缺失DHU臂,其余均可形成典型的三叶草式二级结构。基于库蚊亚科8个种的线粒体基因组系统发育关系为库蚊属Culex+(阿蚊属Armigeres+伊蚊属Aedes)。【结论】分析库蚊亚科的线粒体基因组系统发育关系发现,阿蚊属Armigeres与伊蚊属Aedes亲缘关系较其与库蚊属Culex更近,这与传统的形态分类学结果相吻合。【Aim】 To sequence and analyze the complete mitochondrial genome of Armigeres subalbatus, and to explore the phylogenetic position of Armigeres in Culicinae based on mitochondrial genome sequences. 【Methods】 The nucleotide sequence of the mitochondrial genome of Ar. subalbatus was determined by using PCR amplification and DNA sequencing. The general features and base composition of Ar. subalbatus mitochondrial genome were analyzed, and the phylogenetic relationship of Ar. subalbatus with seven other species of Culicinae was constructed by using maximum likelihood method （ML） and Bayesian inference （BI） based on the nucleotide sequences of concatenated protein-coding genes （PCGs）. 【Results】 The complete mitogenome of Ar. subalbatus is 14 891 bp in length （GenBank accession no.： KY978578）, and contains 37 genes, including 13 PCGs, 22 tRNAs and 2 rRNAs. Its gene organization is consistent with those of the known mitogenomes of Culicidae. The mitogenome has a clear bias in nucleotide composition with a positive AT-skew and a negative GC-skew. All 13 PCGs use the ATN as the initiation codon except COI with TCG, and TAA as the termination codon except COII with the incomplete T. All tRNAs could form the typical cloverleaf structure except tRNASer （AGN）, whose DHU-arm is lacking. The phylogenetic relationship of eight species within Culicinae based on the mitochondrial genomes presents as Culex＋（Armigeres＋Aedes）. 【Conclusion】 The phylogenetic relationship of Culicinae constructed based on the mitochondrial genomes shows that Armigeres is closer related to Aedes than to Culex, which is consistent with the result of the traditional morphologybased taxonomy.

关 键 词：库蚊亚科 阿蚊属 骚扰阿蚊 线粒体基因组 系统发育 

分 类 号：Q969[生物学—昆虫学]