组蛋白去乙酰化抑制剂对拟南芥根尖干细胞微环境的影响  

Inhibitor of Histone Deacetylase Alters the Stem Cell Niche in Arabidopsis Roots

作　　者：胡欢 刘明[1] 吴潜 申聪香[1] 阳成伟[1] 赖建彬 

机构地区：[1]华南师范大学生命科学学院∥广东省植物发育生物工程重点实验室,广州510631

出　　处：《华南师范大学学报（自然科学版）》2018年第1期72-76,共5页Journal of South China Normal University(Natural Science Edition)

基　　金：国家青年科学基金项目(31400314);广州市科技计划项目(201607010377);华南师范大学青年教师科研培育基金(14KJ12)

摘　　要：根系发育是植物生长的重要组成部分,该过程由多种信号转导途径共同调节.组蛋白乙酰化和去乙酰化的动态变化对基因表达具有关键的调控作用,而对其在根发育中功能的研究还不深入.文中通过组蛋白去乙酰化酶抑制剂Trichostatin A(TSA)处理拟南芥,发现其主根生长受到抑制,分生区细胞数目变少.显微观察的结果表明TSA影响了根尖干细胞微环境.根尖微环境调控相关因子SCR/SHR的表达受TSA处理的影响并不明显,而PLT1/PLT2的表达受TSA强烈抑制.对生长素运输途径的分析发现在TSA处理条件下,PIN1表达只受轻微影响,而PIN2表达量明显下调.对突变体pDR5∷GFP的研究结果表明TSA可能引起生长素在根尖的积累和分布变化.综上所述,TSA影响了拟南芥根尖干细胞微环境的维持,表明组蛋白的去乙酰化在根发育过程中发挥着重要作用.A number of signaling pathways are involved in root development,but the role of histone acetylation and deacetylation in this process remains unclear. In this study,Trichostatin A( TSA),an inhibitor for histone deacetylase,was used to determine the effect of histone deacetylation on root development. The root length and the cell number in meristem were reduced by TSA. The result from marker lines suggested that the stem cell niche was disordered during the treatment. The expression levels of SCR and SHR were not altered but those of PLT1 and PLT2 were reduced by TSA. The analysis of the effect on auxin transport showed that the expression of PIN1 was mildly affected but PIN2 was dramatically reduced in the TSA treatment. The result from pDR5∷GFP plants suggested that TSA alters the distribution of auxin in root meristem. In conclusion,TSA has effects on the maintenance of stem cell niche in the root of Arabidopsis,implying the important role of histone deacetylation in root development.

关 键 词：拟南芥 去乙酰化酶 抑制剂 根尖 干细胞微环境 

分 类 号：Q945.4[生物学—植物学]