pIRES2-EGFP-prnd真核表达载体的构建及其在BHK细胞中的表达  

Construction of Eukaryotic Expression Plasmid pIRES2-EGFP-prnd and Its Expression in BHK Cells

作　　者：程艳芬[1,2] 宁章勇[2] 冯翠萍[1] 赵孟孟[2] 安玉甫[2] 吴新涛[2] 

机构地区：[1]山西农业大学食品科学与工程学院,山西太谷030801 [2]华南农业大学兽医学院,广东广州510642

出　　处：《山西农业科学》2018年第3期313-315,319,共4页Journal of Shanxi Agricultural Sciences

基　　金：国家自然科学基金项目(30700603);山西农业大学科技创新项目(2015YJ04)

摘　　要：为探讨叠朊蛋白(Doppel)在正常生理条件及相关疾病中的临床及生物学意义,构建BALB/c小鼠Doppel基因prnd的重组真核表达质粒pIRES2-EGFP-prnd,并转染BHK细胞,IFA检测其表达情况。结果表明,Doppel蛋白通过pIRES2-EGFP-prnd在BHK细胞中表达。试验可为进一步研究Doppel蛋白的结构、正常的生理生化功能及其与相关疾病的关系提供重要工具。To investigate the clinical and biological significance of Doppel in normal physiological conditions and related diseases,the prnd gene of the testis of BALB/c mouse was cloned into the eukaryotic expression vector pIRES2-EGFP.The positive recombinant was designated with pIRES2-EGFP-prnd.After being identified by restriction enzyme digestion and sequencing,the recombinant pIRES2-EGFP-prnd was transfected into BHK cells by liposomes.The expression of the recombinant pIRES2-EGFP-prnd in the BHK cells was observed by fluorescence microscope and IFA.The result showed that the prnd gene was successfully expressed in the BHK cells.It will provide an important tool to further study the structure of Doppel protein,its normal physiological and biochemical functions and its relationship with related diseases.

关 键 词：Doppel蛋白 真核表达载体 转染 IFA 

分 类 号：S852.65[农业科学—基础兽医学]