发根农杆菌菌株和花生品种对发根诱导率的影响  

Effects of Agrobacterium rhizogenes Strains and Peanut Varieties on Induction Rate of Hairy Roots

作　　者：任艳[1] 李双铃[1] 尹亮[1] 崔潇[2] 李磊[2] 石延茂[1] 袁美[1] 

机构地区：[1]山东省花生研究所/农业部花生生物学与遗传育种重点实验室,山东青岛266100 [2]青岛市农业科学研究院,山东青岛266100

出　　处：《山东农业科学》2018年第3期103-106,共4页Shandong Agricultural Sciences

基　　金：山东省农业科学院青年科研基金项目(2015YQN21);科技部"863"项目子课题(2013AA102602);国家自然科学基金项目(31471533)

摘　　要：试验以花育20、花育45、鲁花11无菌花生子叶为材料,采用3×3双因子设计,研究3株发根农杆菌菌株和花生品种对花生发根诱导率的影响。结果表明:发根农杆菌和花生品种均显著影响发根诱导率。花育45的发根诱导率最高,为93.7%,其适宜菌株为A4;花育20的发根诱导率为89.2%,适宜菌株为ATCC15834;鲁花11的发根诱导率为73.4%,适宜菌株为GIM1.141。Three Agrobacterium rhizogenes strains on the induction rate of hairy roots of peanut were investigated by 3 × 3 double-factor design with the cotyledon of sterile plantlets of Huayu 20,Huayu 45,Luhua11 as meterials. The results showed that both Agrobacterium rhizogenes and peanut varieties significantly affected the induction rate of hairy roots. Huayu 45 had the highest induction rate of 93. 7%,and its appropriate strain was A4. The hairy roots induction rate of Huayu 20 was 89. 2%,and the suitable strain was ATCC15834.The hairy roots induction rate of Luhua 11 was 73. 4%,and its fitting strain was GIM1. 141.

关 键 词：发根农杆菌 发根 诱导率 花生品种 

分 类 号：S565.2[农业科学—作物学] S154.39