复方红花酊对白癜风豚鼠模型TNF-α和IL-10表达的影响  

Effect of Compound Safflower Tincture on Expression of TNF-α and IL-10 in Guinea Pig Model of Vitiligo

作　　者：姜丽莎 张国斌[2] 王恩波[2] 张晓东[2] 

机构地区：[1]章丘区人民医院,山东济南250200 [2]解放军第202医院,辽宁沈阳110003

出　　处：《皮肤科学通报》2017年第6期714-721,共8页Dermatology Bulletin

基　　金：辽宁省自然科学基金(201102231)

摘　　要：目的探讨复方红花酊对TNF-α和IL-10的影响及促进黑色素形成作用。方法实验动物随机分为正常对照组、模型组、阳性对照组(复方卡力孜然酊)、复方红花酊高剂量组、中剂量组、低剂量组。建立白癜风皮损模型,并应用相对应药物涂擦治疗,测量其TNF-α和IL-10平均光密度及黑色素颗粒细胞数目,比较其作用。结果复方红花酊高、中剂量组及阳性对照组对皮损有复色作用;与正常对照组比较,模型组TNF-α的表达显著增高,IL-10的表达明显减少,黑色素含量明显降低,差异有统计学意义(P<0.05);与模型组比较,复方红花酊高、中剂量组及阳性对照组中TNF-α的表达明显降低,IL-10的表达明显增高,各组黑色素含量均明显增加,差异有统计学意义(P<0.05);复方红花酊高、中剂量组及阳性对照组中的TNF-α表达水平与黑色素含量呈显著负相关(P<0.05),IL-10表达水平与黑色素含量呈显著正相关(P<0.05)。结论复方红花酊通过调节TNF-α和IL-10的表达水平可影响黑色素的生成,进而对白癜风产生一定的治疗作用。Objectiv Methods Results e To study the compound safflower tincture in vitiligo by observing expres- sion levels of skin tumor necrosis factor-α（TNF-α） , Interleukin-10（ IL- l0） and melanin content. Experimental animals were randomized into the sham group, negative control group, positive control group （Compound Kaliziran Tincture） , compound safflower tincture high-dose group, compound safflower tinc- ture middle-dose group and compound safflower tincture low-dose group. Skin lesion model of vitiligo was established with chemical de- colorization. Different medicine were applied in corresponding groups. Mean optical density of TNF-α and IL-10, and number of melanocytes were measured. Results were analysized with single factor analysis of variance method. High-dose compound safflower tincture group, middle-dose compound saf- flower tincture group and positive control group had repigmentation effects on skin lesions. Compared with normal control group, the expres- sions of TNF-α in shamgroup increased markedly, the expressions of IL- l0 decreased markedly and the contents of melanin decreased markedly, with statistical significance （ P 〈 0.05 ）. Compared with negative control group, the expressions of TNF-α in high-dose compound safflower tincture group, middle-dose compound safflower tincture group and active control group decreased markedly, with statistical significance （ P 〈 0.05 ） ; the expressions of IL-10 and contents of melanin in these three groups in- creased markedly, with statistical significance （ P 〈 0.05 ）. Correlative a- nalysis showed that the expression levels of TNF-oL inhigh-dose compound safflower tincture group, middle-dose compound safflower tincture group and active control group were negatively related to the contents of mela- nin（P 〈0.05）,but the expression levels of IL-10 in these three groups were positively correlated with the content of melanin （P 〈 0.05 ）. Conclusion Compound safflower tincture may affect the f

关 键 词：白癜风 豚鼠 肿瘤坏死因子-Α 白细胞介素-10 黑色素 

分 类 号：R243[医药卫生—中医临床基础] R758.41[医药卫生—中医学]