IL-1β引起食管鳞状细胞癌细胞上皮-间质转化的实验研究  

IL-1 beta induces epithelial mesenchymal-transition in esophageal squamous cell carcinoma cells

作　　者：周剑 刘涛[2] 郑树涛[2] 刘清[2] 陈玉梅 马蓉 谭豆豆[1] 卢晓梅 

机构地区：[1]新疆医科大学附属肿瘤医院,乌鲁木齐830011 [2]新疆医科大学临床医学研究院,乌鲁木齐830011

出　　处：《新疆医科大学学报》2018年第3期274-276,280,共4页Journal of Xinjiang Medical University

基　　金：国家自然科学基金(U1603284;U1303321);新疆维吾尔自治区重大科技专项计划(201430123-1);新疆重大疾病重点实验室开放课题(SKLIB-XJMDR-2016)

摘　　要：目的研究白细胞介素1-β(IL-1β)对食管鳞状细胞癌(ESCC)细胞上皮-间质转化(EMT)的影响。方法在细胞培养基中加入IL-1β,观察IL-1β处理48h后ESCC细胞形态及EMT相关标志物的变化,另以加入PBS的ESCC细胞为阴性对照组。结果 IL-1β处理后ESCC细胞由上皮样形态转变为间质样形态。qRTPCR与Western blot结果显示,IL-1β处理后上皮标志物E-钙黏蛋白(E-cadherin)表达减少,间质标志物波形蛋白(Vimentin)和Slug表达增加,与对照组比较差异有统计学意义(P<0.05)。结论 IL-1β可引起ESCC细胞发生EMT。Objective To investigate the effect of IL-1βon epithelial mesenchymal transition(EMT)in ESCC cells.Methods IL-1βwas added to the cell culture medium,and the morphological changes of ESCC cells and EMT related markers were observed 48 hafter IL-1βtreatment.Cells added PBS was the untreated group.Results The morphology of ESCC cells was altered dramatically from a typical epithelial-like shape of mesenchymal.qRT-PCR and Western blot revealed that IL-1βtreatment resulted in down-regulated the epithelial marker E-cadherin(P <0.05)and up-regulated the mesenchymal marker Vimentin and Slug(P <0.05).Conclusion IL-1βis able to induce EMT in ESCC cells.

关 键 词：食管鳞状细胞癌 白细胞介素1-Β 上皮-间质转化 

分 类 号：R735.1[医药卫生—肿瘤]