Prolonged and highly efficient intracellular extraction of photosynthetic electrons from single algal cells by optimized nanoelectrode insertion  

作　　者：Hyeonaug Hong Yong Jae Kim Myungjin Han Gu YOO Hyun Woo Song Youngcheol Chae Jae-Chul Pyun Arthur R. Grossman WonHyoung Ryu 

机构地区：[1]Department of Mechanical Engineering, Yonsei University, Seou103722, Republic of Korea [2]Yonsei Institute of Convergence Technology and school of In tegrated Technology, Yonsei University, Incheon 21983, Republic of Korea [3]Department of Materials Science & Engineering, Yonsei University, Seou103722, Republic of Korea [4]Department of Electrical & Electronic Engineering, Yonsei University, Seou103722, Republic of Korea [5]Deportment of Plant Biology, Carnegie Institution for Science, Department of Biology, Stanford University, Stanford, CA 94305, USA

出　　处：《Nano Research》2018年第1期397-409,共13页纳米研究（英文版）

摘　　要：Harvesting photosynthetic electrons （PEs） from plant or algal cells can be a highly efficient and environmentally friendly way of generating renewable energy. Recent work on nanoelectrode insertion into algal cells has demonstrated the possibility to directly extract PEs from living algal cells with high efficiencies. However, the instability of the inserted cells limits the practicality of this technology. Here, the impact of nanoelectrode insertion on intracellular extraction of PEs is characterized with the goal of stabilizing algal cells after nanoelectrode insertion. Using nanoelectrodes 〈 500 nm in diameter, algal cells remained stable for over one week after insertion and continued to provide PEs through direct extraction by the inserted nanoelectrodes. After nanoelectrode insertion, a photosynthetic current density of 6 mA.cm-2, which is several fold higher than the current densities attained using approaches based on isolated thylakoid membranes or photosystem I complexes, was observed in the dark and during illumination at various light intensities.Harvesting photosynthetic electrons （PEs） from plant or algal cells can be a highly efficient and environmentally friendly way of generating renewable energy. Recent work on nanoelectrode insertion into algal cells has demonstrated the possibility to directly extract PEs from living algal cells with high efficiencies. However, the instability of the inserted cells limits the practicality of this technology. Here, the impact of nanoelectrode insertion on intracellular extraction of PEs is characterized with the goal of stabilizing algal cells after nanoelectrode insertion. Using nanoelectrodes 〈 500 nm in diameter, algal cells remained stable for over one week after insertion and continued to provide PEs through direct extraction by the inserted nanoelectrodes. After nanoelectrode insertion, a photosynthetic current density of 6 mA.cm-2, which is several fold higher than the current densities attained using approaches based on isolated thylakoid membranes or photosystem I complexes, was observed in the dark and during illumination at various light intensities.

关 键 词：bio-solar energy photosynthetic electrons algal cells NANOELECTRODE cell insertion 

分 类 号：Q26[生物学—细胞生物学] TU831.7[建筑科学—供热、供燃气、通风及空调工程]