LCMS-IT-TOF法分析黄芪赤风汤提取物的化学成分  

Identification of Chemical Constituents in Extract of Huangqi Chifeng Decoction by LCMS-IT-TOF

作　　者：谷雨龙[1] 刘斌[2] 姜艳艳[2] 

机构地区：[1]中央民族大学民族医药教育部重点实验室,北京100081 [2]北京中医药大学中药学院,北京100102

出　　处：《中华中医药学刊》2018年第3期630-637,共8页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81603278);中央高校基本科研基金项目

摘　　要：目的：分析鉴别黄芪赤风汤提取物化学成分。方法：采用高效液相色谱-离子阱-飞行时间质谱法（LCMS-IT-TOF）对黄芪赤风汤提取物化学成分进行分析,采用Venusil MP-C18色谱柱（4.6 mm×250 mm,5μm）,乙腈（A）-水（B）为流动相进行梯度洗脱,电喷雾离子源为质谱离子源,正、负离子检测模式,获得不同化合物的多级质谱数据。结果：根据高分辨质谱数据信息,结合对照品质谱信息及相关文献,共鉴定推断出41个化合物。结论：LCMS-IT-TOF可全面、快速分析黄芪赤风汤提取物中的化学成分,为其质量控制提供参考。Objective： To analyze chemical compositions in the extract of Huangqi Chifeng Decoction. Methods： The liquid chromatography-mass spectrometry-ion trap-time-of-flight（ LCMS-ITTOF） method was applied to analyze the extract of Huangqi Chifeng Decoction. The chromatographic separation was performed on Venusil MP-C18 column（ 4. 6 mm × 250 mm,5 μm） with gradient elution of acetonitrile（ A）-water（ B）. Multistage mass spectrometry data of different compounds was obtained by using electrospray ionization source and monitoring in positive and negative ion modes. Results： Forty-one chemical constituents were identified based on the characteristic MS/MS fragment ions data with chemical standards and published literature. Conclusion： The method is rapid and comprehensive,which is useful for quality control of the extract of Huangqi Chifeng Decoction.

关 键 词：黄芪赤风汤提取物 黄芪 赤芍 防风 高效液相色谱-离子阱-飞行时间质谱法 

分 类 号：R284.1[医药卫生—中药学]