转G10eve基因高抗草甘膦棉花的农艺性状分析  

Agronomic Traits of Transgenic Cotton With High Glyphosate Resistance Conferred by Expressing the G10eve Gene

作　　者：陆国清 王春玲[2] 郝宇琼 郭惠明 黄英金[1] 程红梅[2] 

机构地区：[1]江西农业大学农学院/作物生理生态与遗传育种教育部重点实验室,江西南昌330045 [2]中国农业科学院生物技术研究所,北京100081

出　　处：《核农学报》2018年第2期274-282,共9页Journal of Nuclear Agricultural Sciences

基　　金：国家转基因重大专项(2016ZX08005-004);中国农业科学院科技创新工程

摘　　要：为培育高抗草甘膦棉花,本研究以转G10eve基因棉花为试验材料,通过室内鉴定结合大田试验,筛选高抗、稳定遗传的转基因株系。结果表明,幼苗期喷施不同浓度草甘膦,转基因株系L91抗性水平高于L152,抗性表现稳定,且根、茎、叶间莽草酸积累量差异明显,在叶片中相对含量最高。不同浓度草甘膦处理后,内源EPSPS基因相对表达量呈先升高后降低的趋势,在受体Coker 312中基因相对表达量显著高于转基因株系,表明转G10eve基因可以提高棉花的草甘膦抗性水平。大田试验表明,L91和商业化品种AuR可耐受20 mL·L^(-1)草甘膦。幼苗期喷施草甘膦后,L91株系的株高、果枝数、有效铃数相对于未喷施条件下(对照组)有所增加;AuR的各农艺性状指标与对照组差异不显著;花期喷施不高于20mL·L^(-1)草甘膦,L91和AuR棉花的株高、果枝数、有效铃数均未受抑制,表明,L91株系能够满足生产要求。本研究鉴定筛选得到高抗草甘膦L91转基因株系,为培育抗除草剂棉花新品种提供了种质资源。In this study,the G10 eve transgenic cotton was used to selected high resistance and stable inheritance lines in the lab and field for cultivating new glyphosate-resistant cotton. The results showed that the resistance level of transgenic cotton line 91（ L91） was evidently higher than line L152（ L152） after spraying different concentration of glyphosate at the cotyledon stage,and the resistance was stable. The accumulation of shikimic acid was obvious different in root,sterm and leaf,and the highest was in leaves. After the treatment of glyphosate,the expression of endogenous EPSPS increased firstly and then decreased. The relative expression level of endogenous EPSPS gene in Coker312 was significantly higher than that in transgenic lines,which further demonstrated that transgenic G10 eve gene in cotton could improve the resistance to glyphosate. In the field trials,L91 and AuR（ a commercial varieties） could tolerate 20 mL·L^（-1） glyphosate. When treated with glyphosate at the seedling stage,the plant height,number of branches or number of effective bolls in L91 were increased comparing with the untreated control,while there was no significant difference in those agronomic traits of AuR compared to the control. Moreover,those agronomic traits of L91 and AuR were not inhibited when treated with glyphosate no more than 20 mL·L^（-1） during the flowering phase. These results implied that L91 could meet the requirements in production. L91 with high resistance level of glyphosate was identified and screened in this work,which provided germplasm resources for cultivating new glyphosate-resistant cotton varieties.

关 键 词：转基因 草甘膦 G10eve 抗性 

分 类 号：S562[农业科学—作物学]