猴头菌素分离纯化、结构鉴定及体外活性研究  

Study on the Separation and Purification,Structure Identification and in Vitro Activity of Hericium erinaceus

作　　者：何晋浙 樊鹏 孙培龙 

机构地区：[1]浙江工业大学海洋学院食品与加工安全系,浙江杭州310014

出　　处：《核农学报》2018年第2期318-324,共7页Journal of Nuclear Agricultural Sciences

基　　金：国家高技术研究发展计划(863计划)项目(2014AA022205)

摘　　要：为探究猴头菌功效成分的结构构成及其生物活性,对猴头菌发酵菌丝体中猴头菌素进行分离纯化和结构鉴定,并对其抑菌活性、抗肿瘤活性及刺激神经细胞分泌生长因子能力进行分析。采用乙醇回流提取,并依次通过石油醚、乙酸乙酯萃取得到粗萜物质,粗萜物质经硅胶柱层析和半制备高效液相色谱分离纯化得到组分Fr-3-1,红外光谱及质谱分析表明其为猴头菌素A类似物。采用滤纸片法测定Fr-3-1抑菌活性,MTT法测定其体外抗肿瘤活性,酶联免疫吸附法测定其刺激神经细胞分泌生长因子能力。结果表明,Fr-3-1对金黄色葡萄球菌具有良好的抑菌活性,对人胃癌细胞MGB-523有一定的抑制效果,其最小抑菌浓度(MIC)值分别为0.781 mg·mL^(-1)和453.14μg·mL^(-1)。当浓度为100μg·mL^(-1)时,Fr-3-1促进大鼠星型神经细胞的分泌神经因子浓度为46.63 pg·mL^(-1),显著高于同浓度的肾上腺素。本研究结果为猴头菌作为治疗老年痴呆症和神经衰弱等症状的备选药物及保健食品的开发利用提供了理论依据。In order to study the structure and bioactivity of the functional components of Hericium erinaceus,the mycelium of Hericium erinaceus was isolated and purified with its structure identified. Its antibacterial activity,antitumor activity,ability to stimulate growth factor of nerve cells were also tested. The crude terpene substance was extracted from the extraction of ethanol reflux,petroleum ether,and ethyl acetate. The component Fr-3-1 was isolated and purified by silica gel column chromatography and semi-preparative high performance liquid chromatography of the crude terpene,which was identified as the analogues of erinacine A via infrared spectroscopy and mass spectrometry. The antibacterial activity of Fr-3-1 was determined by filter paper method,and its antitumor activity was measured by MTT assay.Enzyme linked immunoassay was used to evaluate the ability to stimulating the growth of nerve cells. The results indicated that Fr-3-1 had remarkable antibacterial activity against Staphylococcus aureus and had certain inhibitory effect on MGB-523 cancer cells with the MIC respectively of 0. 781 mg·mL^（-1） and 453. 14 μg·mL^（-1）. When the concentration of Fr-3-1 was 100 μg·mL^（-1）,the secretion of growth factor of rat astrocytic cells was 46. 63 pg·mL^（-1）,which was significantly higher than that of epinephrine. All of these results provided experimental evidences that Heriacium erinaceus can act as alternative drugs for the Alzheimer and neurasthenia symptoms diseases,and can promote the development and application as healthy food.

关 键 词：猴头菌素 分离 纯化 抑菌 抗肿瘤活性 

分 类 号：TQ461[化学工程—制药化工] TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]