响应面法优化超声波辅助灵芝黑色素提取工艺  

The Response Surface Method to Optimize the Ultrasonic-assisted Extraction of Ganoderma lucidum Melanin

作　　者：张小林[1] 陈博文[1] 孙晓瑞[2] 骆燕[2] 杜红霞[2] 孟俊龙[2] 常明昌[2] 徐丽婧[2] 

机构地区：[1]山西农业大学园艺学院,山西太谷030801 [2]山西农业大学食品科学与工程学院,山西太谷030801

出　　处：《核农学报》2018年第3期532-538,共7页Journal of Nuclear Agricultural Sciences

基　　金：山西省煤基重大科技攻关项目(FT2014-03-01);山西省高校(2011)黄土高原食用菌提质增效协同创新中心项目

摘　　要：为探究超声波辅助法提取灵芝天然黑色素的最佳工艺条件,本试验以灵芝子实体粉为原材料,以黑色素溶液吸光度为响应值,分别考察Na OH浓度、料液比、超声时间和超声功率4个因素对提取效果的影响,并在单因素试验基础上,通过Box-Behnken响应面分析法优化灵芝天然黑色素的超声波辅助提取工艺。结果表明,响应面法合理可行,最佳提取条件为Na OH浓度1 mol·L^(-1)、料液比1∶39.7g·m L^(-1)、超声功率为162 W和超声时间49.8 min,在此条件下灵芝黑色素吸光度为0.782,与预测值0.783相近。本试验从多角度优化超声波辅助提取灵芝黑色素工艺,为灵芝黑色素的进一步加工提供了理论依据。In order to optimize the extraction process of melanin from Ganoderma lucidum fruiting bodies,influence of Na OH concentration,material-liquid ratio,ultrasonic time and ultrasonic power were investigated with the absorbance of melanin solution as the response value. On the basis of single factor experiment,the optimum processing conditions were determined by Box-Behnken experimental design. The results show that the response surface methodology is reasonable and feasible. The optimal extraction conditions were chosen as 1 mol·L^（-1）,1： 39. 7 g·m L^（-1）,162 W and 49. 8 min for Na OH concentration,solid-liquid ratio,ultrasonication power and time,respectively. The experimental absorbance of the melanin extracted under the optimized conditions was 0. 782,which was in accordance with the predicted value（ 0. 783） of the model. This study optimized the extraction conditions of the Ganoderma lucidum melanin from many aspects and provided a theoretical basis for its processing.

关 键 词：灵芝 黑色素 超声波辅助 提取工艺 响应面 

分 类 号：TS264.4[轻工技术与工程—发酵工程]