利用SSR分析华中、华东五大湖区野生菰的遗传结构与多样性  

Genetic Diversity and Genetic Structure of Zizania latifolia Griseb(Turcz) From Middle-East China Based on SSR Analysis

作　　者：王惠梅[1] 苏晓娜 黄雪雯 王圣子海 熊琦哲 江绍琳[4] 江绍玫[3] 吴建利[1] 

机构地区：[1]中国水稻研究所,浙江杭州310006 [2]西财经大学艺术学院,江西南昌330013 [3]江西财经大学统计学院,江西南昌330013 [4]江西农业大学农学院,江西南昌330045

出　　处：《核农学报》2018年第4期654-664,共11页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金(31460378)

摘　　要：为进一步了解我国野生菰资源的生存现状及演进历程,利用7对SSR分子标记对来自华中、华东5大湖区的26个野生菰居群的遗传多样性和遗传结构进行了分析。结果表明,在物种水平上,华中、华东野生菰具有较丰富的遗传多样性(PPB=92.19%、Ao=1.922、Ae=1.454、He=0.274、I=0.422);各野生菰居群间的遗传相似性系数和遗传距离分别介于0.5569~0.9981和0.0019~0.5854之间,居群间的遗传分化较显著(Gst=0.673)。26个野生菰居群的遗传结构可以分为3组,且各居群间不存在明显的亲缘地理关系。此外,进化分析结果显示,26个野生菰居群具有共同的祖先,在进化上分属两支。本研究结果深化了对我国野生菰资源遗传进化的认识,为野生菰资源开发利用及种质资源保护提供了理论依据。In order to further understand the survival condition and evolution of Z. latifolia in China,seven SSR markers were used for genetic diversity and genetic structure analysis of 26 wild populations from the Middle-East China. The results of POPGENE analysis showed that there was rich genetic diversity among the wild species（PPB = 92. 19%,Ao =1. 922,Ae = 1. 454,He = 0. 274,I = 0. 422,Gst = 0. 673） and the genetic similarity coefficient and genetic distance were between 0. 5569 ~ 0. 9981 and 0. 0019 ~ 0. 5854,respectively. With the genetic structure analysis,26 populations were divided into three groups and there was no significant association between the phylogenetic relationship and the geographic locations in the Z. latifolia populations. The evolution analysis showed that 26 populations were separated from the same ancestor into two branches. This study deepens our understanding about the genetic evolution of Z.latifolia in the Middle-East China and provides certain theoretical basis for protection of the wild rice germplasm resource.

关 键 词：菰 SSR 遗传多样性 遗传结构 

分 类 号：Q943[生物学—植物学]