小麦Glu-B1x7沉默基因序列分析及对加工品质的影响  

Sequence Analysis of a Silent Glu-B1x7 Gene and Its Effect on Processing Quality

作　　者：张平平[1] 张瑜[1] 王化敦[1] 宋桂成 姚金保[1] 马鸿翔[1] 

机构地区：[1]江苏省农业科学院/江苏省农业生物学重点实验室/江苏省现代作物生产协同创新中心,江苏南京210014

出　　处：《核农学报》2018年第4期708-714,共7页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金(31671690);江苏省自然科学基金(BK20161375);国家重点研发计划(2016YFD0100502);国家小麦产业技术体系(CARS-03)

摘　　要：小麦籽粒高分子量谷蛋白亚基(HMW-GS)Glu-B1x7与加工品质密切相关。本研究创制了以宁麦9号为背景的Glu-B1x7亚基缺失系,克隆并分析Glu-B1x基因,评价该基因对弱筋小麦加工品质的影响。结果表明,该基因编码区第514位发生了C/G到T/A转换,相应的第514、第515和第516位碱基组成的三联密码子由CAA替换为终止密码子TAA。与野生型宁麦9号相比,具有Glu-B1x7亚基缺失的品系籽粒硬度和蛋白质含量无显著变化,水溶剂保持力和乳酸溶剂保持力均降低,部分品系降低幅度达显著水平。HMW-GS总量和高分子量谷蛋白/低分子量谷蛋白比显著降低,和面仪和面时间显著降低,糖酥饼干直径显著增加。Glu-B1x7基因沉默在宁麦9号背景下可提高弱筋小麦加工品质,该等位变异可用于优质弱筋小麦培育。Glu-B1x7,a high molecular glutenin subunits（HMW-GS）,in wheat gain was highly related with processing quality. In order to clarify the effect of Glu-B1x7 on the processing quality of weak-gluten wheat,genotypes with Glu-B1x deletion were created under the background of Ningmai 9. As a result,C/G to T/A transition was observed at position514 of coding sequence in Glu-B1x null gene,which resulted in one new TAA stop codon,and consequently Glu-B1x deletion. Contrast wild-type Ningmai 9,no significant differences were observed in kernel hardness and protein content in Glu-B1x deletion lines,while water solvent retention capacity（WSRC） and lactic acid solvent retention capacity（LASRC） decreased. Significant lower content of HMW-GS,and lower ratio of HMW to LMW,and mixograph mixing time were also showed in Glu-B1x deletion lines. Subsequently,Glu-B1x deletion lines produced larger cookie diameter than wild-type Ningmai 9. This study showed that Glu-B1xnull could improve the weak-gluten wheat processing quality under the Ningmai 9 background,which could be used to breed high qualty weak-qulten wheat.

关 键 词：高分子量谷蛋白亚基 基因序列 弱筋小麦 加工品质 

分 类 号：S512.1[农业科学—作物学]