花生种子白藜芦醇含量QTL定位分析  

QTL analysis of resveratrol content in peanut seeds

作　　者：喻博伦 黄莉[1] 邱西克 郭建斌[1] 姜成红[1] 蔡岩[1] 廖伯寿[1] 姜慧芳[1] 陈玉宁[1] 刘念[1] 陈伟刚[1] 吕建伟[1] 宋延滨 罗怀勇[1] 

机构地区：[1]中国农业科学院油料作物研究所/农业部油料作物生物学与遗传育种重点实验室,湖北武汉430062

出　　处：《中国油料作物学报》2018年第1期35-41,共7页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金项目(31601340);农业部农作物种质资源保护项目(2017NWB033);花生种质资源平台项目(NICGR2017-014);国家现代农业产业技术体系建设专项(CARS-14-花生种质资源评价);湖北省自然科学基金项目(2017CFB540)

摘　　要：本研究利用"中花10×ICG 12625"衍生的RIL群体共140个家系及其亲本为材料,通过高效液相色谱检测各家系在2014年及2015年收获的花生种子中的白藜芦醇含量。结果表明,RIL群体白藜芦醇含量变异范围为38.27~352.08μg/kg。通过两年重复检测,获得稳定高白藜芦醇含量材料4份(QT0339、QT0369、QT0450和QT0454),含量为143.90~215.60μg/kg,在2014年环境中,这四份材料白藜芦醇含量分别超过高值亲本32.31%、5.80%、86.46%和79.52%,在2015年环境中白藜芦醇含量分别超过高值亲本106.04%、49.78%、7.90%和52.87%。利用前期通过该群体构建的遗传连锁图谱,应用WinQTLCart 2.5软件定位到花生种子白藜芦醇含量相关QTL13个,贡献率在2.2%~7.4%之间,其中qRB8.1a以及qRB8.1b为两年环境中重复检测到的QTL。本文研究结果为培育白藜芦醇含量高的花生新品种提供理论依据。Resveratrol is an important component of phytoalexins,and it affects human cardiovascular system.In this study,the resveratrol content in seeds of 140 lines in an RIL population（ Zhonghua 10 × ICG 12625） was detected by high performance liquid chromatography（ HPLC） in two environments. The result showed that the variation resveratrol contents in the RIL population ranged from 38. 27μg/kg to 352. 08μg/kg. Four Lines（ QT0339,QT0369,QT0450 and QT0454） showed stable higher resveratrol contents than the high value parent in the two environments,ranging from 143. 90μg/kg to 215. 60μg/kg. The resveratrol contents of these lines were higher than that of the high value parent by 32. 31%,5. 80%,86. 46% and 79. 52% in 2014 environment and 106. 04%,49. 78%,7. 9% and 52. 87% in 2015 environment,respectively. A total of 13 QTLs related to resveratrol content in peanut seeds were detected by WinQTLCart 2. 5 software,and showed phenotypic variations of 2. 2 - 7. 4%. Notably,qRB8. 1 a and qRB8. 1 b were repeatedly detected in both environments. The results of this study provided a theoretical basis for breeding new peanut varieties with high resveratrol content.

关 键 词：白藜芦醇 花生 QTL定位 

分 类 号：S565.2[农业科学—作物学]