Preparation of dry flowable formulations of Clonostachys rosea by spray drying and application for Sclerotinia sclerotiorum control  

作　　者：WU Hong-qu SUN Li-li LIU Fang WANG Zhi-ying CAO Chuan-wang 

机构地区：[1]College of Forestry, Northeast Forestry University, Harbin 150040, P.R.China [2]Hubei Biopesticide Engineering Research Centre, Wuhan 430064, P.R.China

出　　处：《Journal of Integrative Agriculture》2018年第3期613-620,共8页农业科学学报（英文版）

基　　金：supported by grants from the Fundamental Research Funds for the Central Universities, China (2572016DA02);the National Natural Science Foundation of China (31570642);the Science and Technology Innovation and Entrepreneurship Projects of Returned Overseas Personnel in Jilin Province, China (2013-36)

摘　　要：A dry flowable formulation of Clonostachys rosea with fungicidal activity against Sclerotinia sclerotiorum was prepared by spray drying. The formulation was optimized by a four-factor, three-level orthogonal experiment to screen inert ingredients and spray-drying conditions. The optimal dry flowable formulation of C. rosea included 30% C. rosea （ratio of conidia powder and its fermentation broth is 1：3）, 3% Morwet EFW, 4% K12, 10% Morwet D425, 9% sodium salt of polynaphthalene sulphonic acid （NNO）, 5% croscarmellose sodium, 5% （NH4）2SO4, 0.5% carboxymethyl cellulose sodium （CMC-Na）, 1% oxalic acid and palygorskite （carrier） up to 100%. The formulation exhibited good physical characteristics, such as high dispersibility, viability and a long shelf life. Plate antagonism tests and pot trials indicated that the dry flowable formulation was very effective against S. sclerotiorum, with control efficiency of up to 88.30%. This dry flowable formulation of C. rosea is a new potential commercial fungicide for spray drying to control S. sclerotiorum.A dry flowable formulation of Clonostachys rosea with fungicidal activity against Sclerotinia sclerotiorum was prepared by spray drying. The formulation was optimized by a four-factor, three-level orthogonal experiment to screen inert ingredients and spray-drying conditions. The optimal dry flowable formulation of C. rosea included 30% C. rosea （ratio of conidia powder and its fermentation broth is 1：3）, 3% Morwet EFW, 4% K12, 10% Morwet D425, 9% sodium salt of polynaphthalene sulphonic acid （NNO）, 5% croscarmellose sodium, 5% （NH4）2SO4, 0.5% carboxymethyl cellulose sodium （CMC-Na）, 1% oxalic acid and palygorskite （carrier） up to 100%. The formulation exhibited good physical characteristics, such as high dispersibility, viability and a long shelf life. Plate antagonism tests and pot trials indicated that the dry flowable formulation was very effective against S. sclerotiorum, with control efficiency of up to 88.30%. This dry flowable formulation of C. rosea is a new potential commercial fungicide for spray drying to control S. sclerotiorum.

关 键 词：Clonostachys rosea dry flowable formulation Sclerotinia sclerofiorum spray drying 

分 类 号：S482.2[农业科学—农药学]