土壤中单一及复合抗生素的降解及微生物响应  

Behavior and ecotoxicity of single and mixed antibiotics in soils:Degradation and changes in microbial community

作　　者：阮琳琳 林辉[2] 马军伟[2] 孙万春[2] 俞巧钢[2] 符建荣[2] 

机构地区：[1]浙江师范大学生命与化学科学学院,浙江金华321004 [2]浙江省农业科学院环境资源与土壤肥料研究所,浙江杭州310021

出　　处：《中国环境科学》2018年第3期1081-1089,共9页China Environmental Science

基　　金：国家自然科学基金资助项目(31672234;41401542;31670110);浙江省科公益技术应用项目(2017C32005;LGN18D010005)

摘　　要：基于LC-MS/MS、Micro RESPTM等技术,对比了磺胺二甲嘧啶(SM2)和强力霉素(DOX)在单一和共存条件下,在青紫泥和小粉土中的短期降解及土壤微生物代谢特征.结果表明,单一条件下,小粉土中SM2和DOX的3d和7d降解率总体高于青紫泥,SM2和DOX共存条件下可对土壤中二者的短期降解产生一定抑制,这种抑制作用在小粉土中更为明显.抗生素对土壤微生物代谢的影响与其赋存时间及土壤类型有关.在供试浓度10~20mg/kg下,SM2和DOX在单一和复合条件下总体是促进土壤微生物代谢,但影响随赋存时间增加而减弱.青紫泥中,SM2和DOX共存下的土壤微生物代谢多样性高于二者单一存在,且青紫泥中DOX浓度与土壤代谢活性和微生物群落代谢多样性呈显著正相关,而小粉土中无类似的现象,但SM2与DOX复合污染对土壤微生物代谢的影响总体源于DOX的作用.This work studied the short-term degradation of antibiotics in a purple clay and a silt soil after individual andcombined application of sulfadimidine （SM2） and doxycycline （DOX）; the responses of soil microbial communitymetabolism to different antibiotics perturbation were also compared; Liquid chromatography-tandem mass spectrometry（LC-MS/MS） and MicroRESPTM technology were used herein. Results indicated that the degradation rates of both SM2and DOX on the 3rd and 7th day in the silt soil were higher than that in the purple clay. Compared with that in singleantibiotic pollution systems, co-existence of DOX and SM2 in combined antibiotics pollution system inhibited theshort-term degradation of both antibiotic types in the two soil types; this inhibition was stronger in the silt soil. Both thesingle and mixed antibiotics in this work enhanced the metabolisms of soil microbial community with antibiotic exposureconcentration from 10mg/kg to 20mg/kg, whereas the impacts of antibiotics decreased as the exposure time of theantibiotic increased. In the purple clay, the mixed antibiotic treatment showed significantly higher metabolic diversity ofsoil microbial community than all the single antibiotic treatments; its metabolic activity and diversity of soil microbialcommunity correlated positively and significantly with the DOX concentration. However, similar phenomena were notseen in the silt soil. Meanwhile, the effect of mixed antibiotic on soil microbial community metabolism was mainly due tothe function of DOX herein.

关 键 词：青紫泥 小粉土 磺胺二甲嘧啶 强力霉素 降解 土壤微生物 MicroRESPTM 

分 类 号：X53[环境科学与工程—环境工程]