静态磁场对生物滴滤池强化的研究——以三氯乙烯废气的去除及细菌群落结构研究为例  

The strengthening effect of a static magnetic field on biotrickling filter——Study on trichloroethylene removal and bacterial community

作　　者：权跃[1,2] 吴昊[2] 尹振浩 郭春雨 尹成日[2,3] 

机构地区：[1]延边大学农学院环境科学系,吉林延吉133002 [2]延边大学理学院化学系,吉林延吉133002 [3]延边大学分析测试中心,吉林延吉133002

出　　处：《中国环境科学》2018年第3期1099-1108,共10页China Environmental Science

基　　金：国家自然科学基金(21662038)

摘　　要：构建了实验室规模磁场强化的生物滴滤池(MF-BTF),并设对照生物滴滤池(S-BTF),以火山石为载体,接种活性污泥,处理目标废气三氯乙烯(TCE).研究了不同磁场强度下,2种生物滴滤池在稳定运行期间对TCE的去除效果,并采用高通量测序技术对滴滤池中细菌群落结构与功能进行了分析.实验结果表明:在好氧条件下,当空床停留时间(EBRT)为202.5s,苯酚浓度为0.20g/L,TCE浓度范围为53.6~337.1mg/m^3时,对TCE的去除效果依次为MF-BTF(60.0m T)>MF-BTF(30.0m T)>S-BTF(0.0m T)>MF-BTF(130.0m T),其去除率(RE)和最大去除容量(EC)分别为92.2%~45.5%,2656.8mg/(m^3·h);89.8%~37.2%,2169.1mg/(m^3·h);89.8%~29.8%,1967.7mg/(m^3·h);76.0%~20.8%,1697.1mg/(m^3·h).高通量测序结果表明:磁场强度为60.0m T时,细菌群落均匀度(Shannon index,Simpson index)及丰富度(OTUs,Chao1,ACE)均小于0.0m T的情况;但是60.0m T下优势门、纲、属为Proteobacteria,Gammaproteobacteria,Acinetobacter,丰度明显大于0.0m T,丰度分别为73.3%,36.8%,34.7%;69.6%,18.2%,10.9%.实验结果表明合适的磁场强度可以增大优势菌群的丰度,提高对TCE的去除效果.本实验为提高生物滴滤池的工业废气处理效果提供了有效的借鉴,具有广泛的应用前景.A laboratory-scale biotrickling filter combined with a magnetic field （MF-BTF） and a single BTF （S-BTF）packed with laver rocks were set up to treat trichloroethylene （TCE） gas through inoculation of activated sludge. Theinfluences of different MF intensities were investigated and high-throughput sequencing was studied to bacteriacommunity and functions. The results showed that in aerobic conditions, with 0.20g/L of phenol, 53.6 to 337.1mg/m3 ofTCE, and empty bed residence times （EBRT） of 202.5s, the performances followed the order MF-BTF （60.0mT） 〉MF-BTF （30.0mT） 〉 S-BTF （0.0mT） 〉 MF-BTF （130.0mT）, and the removal efficiencies （REs） and maximumelimination capacities （ECs） corresponded to： 92.2%~45.5%, 2656.8mg/（m3？h）; 89.8%~37.2%, 2169.1mg/（m3？h）;89.8%~29.8%, 1967.7mg/（m3？h）; 76.0%~20.8%, 1697.1mg/（m3？h）, respectively. High-throughput sequencing indicatedthat the bacterial diversity was lower, whereas the relative abundances of predominant bacteria： Proteobacteria,Gammaproteobacteria, Acinetobacter were higher in MF-BTF （60.0mT： 73.3%, 36.8%, 34.7%） than that in S-BTF （0mT：69.6%, 18.2%, 10.9%）. Results confirmed that a proper MF could improve TCE removal performance in BTF.

关 键 词：磁场 生物滴滤池 三氯乙烯 细菌群落 高通量 

分 类 号：X505[环境科学与工程—环境工程]