质谱直接定量分析技术的应用进展  

Advances in Direct Quantification Analysis by Mass Spectrometry

作　　者：李中权 张芳[2] 苏越 郭寅龙[2] 

机构地区：[1]上海中医药大学,中医方证与系统生物学研究中心,上海201203 [2]中国科学院上海有机化学研究所,上海有机质谱中心,上海200032

出　　处：《质谱学报》2018年第2期129-140,共12页Journal of Chinese Mass Spectrometry Society

基　　金：国家自然科学基金(21572134); 国家科技支撑计划(2015BAK45B01)资助

摘　　要：质谱直接定量分析指待测样品在引入质谱离子源之前,不经色谱分离,直接利用质谱信号对分析物进行含量测定的分析技术。该技术在很大程度上缩短了样品的检测周期,能够满足快速在线、原位分析的需求,目前已在质谱成像、单细胞分析以及在线反应监测等领域展现出广阔的应用前景。本工作综述了与质谱直接定量分析有关的质谱技术、样品处理方法以及相关应用,并展望质谱定量分析的发展趋势。Direct quantification analysis by mass spectrometry refers to the technique that sample is quantified directly by mass spectrometry without chromatographic separation before ionization.Compared to the traditional chromatographic methods,this technique could significantly shorten analytical time,and then further to meet the rapid,online monitoring and in situ analysis demands.At present,new publications have continuously appeared and widened the application areas.This review provided a snapshot of the field and mainly focused on the mass spectrometric techniques and sample pretreatment strategies.From the technical aspect,ambient desorption ionization MS has made a great breakthrough for direct quantitative analysis.Various samples could be directly desorbed and ionized in the open air conditions of the laboratory or in their natu-ral environment.It typically required little or no sample preparation and offers a muchsimplified work flow prior to MS analysis.Certainly,ambient MS techniques have been successfully coupled to mass spectrometers equipped with different high-resolution mass analyzers,such as time of flight（TOF）,Orbitrap and ion cyclotron resonance（ICR）.The ability to provide accurate mass values and isotope patterns makes feasible the unequivocal identification of compounds from MS data.Sometimes,in order to extract the maximum information,hybrid instruments such as triple-quadrupole mass analyzer（QQQ）and quadrupole-TOF possessing multiple scan modes for further identification and quantification analysis is necessary.From the pretreatment aspect,there are two main strategies for direct quantification analysis by MS.The first one is trying to improve the signal response in the mass spectrometer by utilizing the derivative reagent.At the same time,derivatization using stable isotope labeling such as N-alkylpyridinium isotope quaternization could also introduce the internal standard for the accurate quantification.The second strategy is the extraction technique significantly reducing matrix int

关 键 词：质谱直接定量分析 敞开式离子化 前处理 

分 类 号：O657.63[理学—分析化学]