中华散尾鬼笔提取工艺响应面优化及其粗提物毒力测定  

Optimization of Extracts from Lysurus mokusin Using Response Surface Methodology and Their Toxicity Test

作　　者：林连男[1] 王瑞琦 张国珍[1] 王亚军[1] 张晓波 张国财[1] 

机构地区：[1]东北林业大学林学院,哈尔滨150040

出　　处：《吉林农业大学学报》2018年第1期80-84,共5页Journal of Jilin Agricultural University

基　　金：中央高校基本科研业务费专项资金项目(2572016AA03);哈尔滨市应用技术研究与开发项目(2016RAXXJ035);黑龙江省森工总局科研项目(sgzj T2015005)

摘　　要：采用超声提取法,以超声时间、乙醇浓度、提取温度、料液比、超声功率5因素进行单因素提取试验,而后选择对中华散尾鬼笔粗提物提取率有显著影响的3因素进行响应面分析,优化出中华散尾鬼笔粗提物的最佳提取工艺。试验结果表明:在料液比1∶80、乙醇体积分数60%、超声时间80 min时,提取率最高,为31.21%;以舞毒蛾3龄幼虫为供试昆虫,用中华散尾鬼笔粗提物进行毒力测定,试验结果表明,中华散尾鬼笔粗提物对舞毒蛾3龄幼虫的校正死亡率为59.77%。Lysurus mokusin crude extract（ LMCE） from Lysurus mokusin by ultrasonic method was used as an index in this test. The effects of different ultrasonic time,ethanol concentration,ultrasonic power and solid-liquid ratio on extracting rate of LMCE were investigated by single factor experiment,then the three factors,ultrasonic time,ultrasonic power and solid-liquid ratio,were chosen to determine the optimum process parameters in the response surface test. The results showed that under the conditions of 80 min of ultrasonic time,60% ethanol and 1 ∶ 80（ g ∶ m L） of solid-liquid ratio,the extraction rate of LMCE was the highest（ 31. 21%）. The effects of LMCE on gypsy moth larvae were studied. The results showed that the adjusted mortality for the larvae of gypsy moth larvae was 59. 77%.

关 键 词：中华散尾鬼笔 舞毒蛾 响应面优化 提取工艺 毒力测定 

分 类 号：TQ458[化学工程—农药化工] Q949.329[生物学—植物学]