Kisspeptin对牛卵巢颗粒细胞孕酮分泌的影响  

Effects of Kisspeptin on Progesterone Secretion of Bovine Granulosa Cells

作　　者：刘红羽[1] 吕嘉琦 郭乐薇 王军[1] 

机构地区：[1]吉林农业大学动物科学技术学院,长春130118 [2]东北师范大学附属中学,长春130000

出　　处：《吉林农业大学学报》2018年第1期99-102,共4页Journal of Jilin Agricultural University

基　　金：中国博士后基金项目(2015M581408);国家自然科学基金青年基金项目(31402073)

摘　　要：为明确Kisspeptin对牛卵巢颗粒细胞孕酮分泌的作用,采用荧光定量PCR和ELISA方法研究了Kisspeptin对牛卵巢颗粒细胞孕酮分泌的影响。结果表明:Kisspeptin对牛卵巢颗粒细胞的孕酮分泌有促进作用,且在一定范围内呈剂量依赖性。100 nmol/L Kisspeptin-10(Kp-10)处理颗粒细胞24 h后,显著增加了孕酮的分泌(P<0.05),而10 nmol/L和1 000 nmol/L Kp-10处理组对孕酮的分泌无显著影响(P>0.05)。100 nmol/L Kp-10可显著提高孕酮合成的关键基因3β-HSD和St AR的mRNA表达水平(P<0.01)。研究结果提示,Kisspeptin可促进牛卵巢颗粒细胞孕酮的分泌,推测这一作用可能通过上调孕酮合成关键基因表达来实现。In order to investigate the effects of Kisspeptin on progesterone secretion of bovine ovarian granulosa cells,fluorescence quantitative PCR and ELISA were used to study effects of Kisspeptin on progesterone secretion of bovine ovarian granulosa cells. The results showed that Kisspeptin could promote the secretion of progesterone in bovine granulosa cells and showed dose dependence within a certain range. 100 nmol/L Kisspeptin-10（ Kp-10） treatment for 24 h significantly increased progesterone secretion（ P〈0. 05）,while 10 nmol/L and 1 000 nmol/L of Kp-10 treatment had no significant effect on the secretion of progesterone（ P〉0. 05）. 100 nmol/L of Kp-10 treatment significantly increased the level of mRNA expression of key genes of progesterone synthesis 3 beta-HSD and St AR（ P〈0. 01）. The results showed that Kisspeptin could promote the secretion of progesterone in bovine granulosa cells probably by upregulating expression of key genes of progesterone synthesis.

关 键 词：KISSPEPTIN 颗粒细胞 孕酮 

分 类 号：S823.3[农业科学—畜牧学]