BIOLOG与微量热耦合研究提高红壤微生物代谢活性的碳源因子  

Use of BIOLOG and Microcalorimetry in Combination to Study Factors of Carbon Sources Stimulating Metabolic Activity of Soil Microbe in Red Soil

作　　者：徐江兵 王艳玲[1] 刘明 陈美君[1] 林先贵 

机构地区：[1]南京信息工程大学,应用气象学院生态气象环境研究中心,南京210044 [2]土壤与农业可持续发展国家重点实验室(中国科学院南京土壤研究所),南京210008

出　　处：《土壤学报》2018年第1期203-212,共10页Acta Pedologica Sinica

基　　金：国家自然科学基金项目(41501264,41571286)资助

摘　　要：土壤微生物是土壤中重要的活性组成部分,研究土壤微生物代谢活性对于理解农业措施对农业生态系统的影响至关重要。已有研究表明,将BIOLOG与微量热耦合可揭示中性及碱性土壤中影响代谢活性的碳源因子,但对于酸性土壤鲜有报道。选取一种典型的酸性土壤——红壤为研究对象,采用BIOLOG及微量热技术研究施肥后红壤微生物的碳源底物利用能力及热动力学变化情况,进一步耦合两种技术探讨提高红壤微生物活性的碳源因子。结果表明,有机无机肥配施能显著提高红壤微生物的碳源底物利用能力,改变对碳源的偏好,增强其热代谢活性;但某些引起有机无机肥配施处理分异的碳源未对红壤微生物的热代谢活性产生促进作用,相反产生抑制效果,说明BIOLOG具有一定局限性;L-精氨酸和糖原提高红壤微生物的热代谢活性,其原因在于这两种碳源可能提高红壤中氮、磷循环相关的微生物活性。因此,在红壤中施用富含该类碳源的有机肥对提高红壤微生物活性、提升红壤地力具有积极意义。【Objective】Soil microorganisms are important active components in the soil. To understand impacts of farming practices on agro-ecosystems,it is of utmost importance to unfold researches on soil microbial metabolic activity in the farmland. The technique of BIOLOG Eco micro-plate assay is a conventional tool to evaluate soil microorganisms＇ ability of utilizing carbon of one solesource,and though having some limitations,could provide clues to source of the carbon utilized by soil microbes relative to farming practice,while microcalorimetric technique is another means to characterize soil microbial activity by measuring heat dissipation and one of high precision. However,being a non-specific technique,it could,only with the support of specific techniques like BIOLOG,provide more explicit information about functions of soil microbe. Previous studies done by this group of researchers reported that BIOLOG could be used in couple with microcalorimetry to detect the factors of carbon source influencing metabolic activity of the soil microbes in the acid and neutral soils. However,little information has so far been reported about this issue in acidic soils. 【Method】In this study,samples of red soil,a typical type of acid soil,were collected for exploration of ability of the soil microbes to utilize carbon substrates and microcalorimetric dynamics with the technique of BIOLOG coupled with microcalorimetric means in soils applied with chemical fertilizers and organic manure in combination（Treatment OM）. Based on the information of carbon source preference of the soil microbes in the red soil obtained via principle component analysis（PCA）of the BIOLOG assay,evaluation was further performed of effects of those carbon substrates on thermometabolism of the soil microbes in Treatment OM and Treatment CK（no fertilization）.【Result】Results show that Treatment OM significantly increased AWCD（average well color development）on the BIOLOG microplate as compared to Treatment CK. PCA indicates that Treatmen

关 键 词：施肥 有机无机肥配施 糖原 精氨酸 

分 类 号：S154[农业科学—土壤学]