Repeated exposure to moxa-burning smoke: its acute and chronic toxicities in rats  

作　　者：Han Li Liu Changxi Liu Ping Hu Hai Yang Jia Cai Hong Lim Minyee Zhu Maoxiang Pan Xiujie Huang Jian Cui Yingxue Lao Lixing Zhao Baixiao 

机构地区：[1]Shool of Chinese medicine, Beijing University of Chinese Medicine [2]Healthcare Management Center, Beijing Electric Power Hospital [3]Department of Acupunture, Beijing Hospital of Acupuncture and Moxibustion [4]Shool of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine [5]Department of Traditional Chinese Medicine,Chongqing Yubei District Hospital of Traditional Chinese Medicine [6]Institute of Radiation Medicine,China Academy of Military Medical Science [7]Department of Acupunture, Beijing Dong Fang Hospital [8]Department of Acupunture, Beijing Hospital of Traditional Chinese Medicine [9]School of Chinese Medicine, The University of Hong Kong

出　　处：《Journal of Traditional Chinese Medicine》2018年第1期67-75,共9页中医杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China:effect of Moxibustion on"Longevity Gene"SIRT1 Regulation in Aged Mice(No.81403449);NSFC Project:effect of Moxibustion and its Therapeutic Factors on Mitochondrial Energy Metabolism and Apotosis Pathyway in APP/PS1Transgenic Alzheimer's Disease Mouse Model(No.8157-4068);National Program on Key Basic Research Project(973 Program):the Study of Components and Mecha-nisms of Artemisia and the Product and Moxibustion and Safety Evaluation(No.2009CB522906)

摘　　要：OBJECTIVE： To assess toxicities of the air in Chinese medicine clinics polluted by moxa-burning smoke due to moxibustion-derived burning products （MBP）. METHODS： Both acute and chronic toxicity studies were conducted. For the acute toxicity study, five groups of Wistar rats （n = 16/group, male： female = 1 ： 1） were exposed to five different concentrations （95%, 90%, 85%, 80% and 75%, respectively） of MBP for 2 h. For the chronic toxicity study, another three groups of male rats （n = 21/group） were ex- posed to MBP in three concentrations （10%, 40% and 70%, respectively） and one control group ex- posed to clean air 20 min/d for 144 d. Routine ex- aminations were performed and analyzed by analy- sis of variance and dose-response relationship. RESULTS： In the acute toxicity study, the number of dead rats in the 95%, 90%, 85%, 80% and 75% groups were 16, 13, 7, 6 LDS0 of 86.274% after or and 3, respectively, with during the 2 h exposure. In the chronic toxicity study, MBP exposure induced a decline in activity of the rats. Rats in the 10% group showed no signs of toxicity, while those in the 40% MBP group showed toxicity effects on the body weights （P 〈 0.05） and lung. Rats in the 70% MBP group also presented with reversible damage in the blood coagulation system （P 〈 0.05）. CONCLUSION： Exposure to 10% MBP, which is equivalent to 27.45 mg/m^3 was under the critical threshold for male rats＇safety. Exposure to MBP above that limit induced lung damage. MBP in clinics need to be reduced to a safe level with enhanced ventilation.OBJECTIVE: To assess toxicities of the air in Chi-nese medicine clinics polluted by moxa-burning smoke due to moxibustion-derived burning products(MBP).METHODS: Both acute and chronic toxicity studies were conducted. For the acute toxicity study, five groups of Wistar rats(n = 16/group, male: female =1∶1) were exposed to five different concentrations(95%, 90%, 85%, 80% and 75%, respectively) of MBP for 2 h. For the chronic toxicity study, another three groups of male rats(n = 21/group) were exposed to MBP in three concentrations(10%, 40%and 70%, respectively) and one control group exposed to clean air 20 min/d for 144 d. Routine examinations were performed and analyzed by analysis of variance and dose-response relationship.RESULTS: In the acute toxicity study, the number of dead rats in the 95%, 90%, 85%, 80% and 75%groups were 16, 13, 7, 6 and 3, respectively, with LD50 of 86.274% after or during the 2 h exposure.In the chronic toxicity study, MBP exposure induced a decline in activity of the rats. Rats in the10% group showed no signs of toxicity, while those in the 40% MBP group showed toxicity effects on the body weights(P < 0.05) and lung. Rats in the70% MBP group also presented with reversible damage in the blood coagulation system(P < 0.05).CONCLUSION: Exposure to 10% MBP, which is equivalent to 27.45 mg/m3, was under the critical threshold for male rats' safety. Exposure to MBP above that limit induced lung damage. MBP in clinics need to be reduced to a safe level with enhanced ventilation.

关 键 词：SMOKE MOXIBUSTION Environmental exposure Toxicity tests 

分 类 号：R245[医药卫生—针灸推拿学]