乙肝表面抗原核酸疫苗的构建及其与宿主染色体整合的可能性分析  

Construction of hepatitis B S antigen DNA vaccine and its potential integration into host cell genome

作　　者：周凤娟[1] 胡振林[1] 戴建新[1] 孙树汉[1] 

机构地区：[1]第二军医大学基础医学部医学遗传学教研室,上海200433

出　　处：《第二军医大学学报》2002年第8期816-818,共3页Academic Journal of Second Military Medical University

基　　金：国家" 86 3"计划资助项目(2 0 0 1AA2 13111)

摘　　要：目的 :构建乙肝表面抗原 DNA疫苗 ,并探讨其在实验动物体内整合到宿主细胞基因组上的可能性。 方法 :采用PCR法扩增乙肝病毒的 S基因片段后装入 p VAX载体 ,并以此为候选疫苗免疫 (BAL B/c)小鼠 ,在不同时间、不同部位取其组织抽提基因组 DNA,用 PCR检测 DNA整合情况。结果 :构建了乙肝核酸疫苗 p VAX- HBs Ag。免疫接种该疫苗第 3周时在小鼠肌肉、肝脏、脾脏、胸腺均有不同量的游离质粒存在 ;第 6周除在股四头肌可检测到外 ,其余组织中均为阴性 ;第 12、18周时检测所有组织中均为阴性。 结论 :构建的 p VAX- HBs Ag DNA疫苗在小鼠体内无染色体整合。Objective:To construct DNA vaccine for hepatitis B S(HBS) antigen and to study its potential integration into host cell genome in animals. Methods:S gene of hepatitis B virus was amplified by PCR and cloned into DNA vaccine vector pVAX. Mice were immunized by the constructed candidate for DNA vaccine. The potential integration of DNA was checked with PCR. Results: Hepatitis B virus DNA vaccine (pVAX HBsAg) was constructed. In the third week after immunized by the vaccine there were different amount of free plasmids in muscle, liver, spleen and thymus. In the 6th week plasmid DNA could only be detected in quadriceps. All tissues were negative in the 12th and 18th week. Conclusion: No genomic integration of constructed DNA vaccine (pVAX HBsAg) is detected in the immunized mice.

关 键 词：乙型肝炎表面抗原 核酸疫苗 DNA疫苗 宿主 染色体整合 

分 类 号：R512.62[医药卫生—内科学] R392－33[医药卫生—临床医学]