骨小梁仿生微结构的解析与构建  

Analysis and Construction of Trabecular Bone Bionic Microstructure

作　　者：赵小文 白雪岭[2] 张鹏[2] 张东锋 穆林 

机构地区：[1]深圳市艾科赛龙科技股份有限公司,深圳518053 [2]中国科学院深圳先进技术研究院,深圳518055

出　　处：《集成技术》2018年第1期1-10,共10页Journal of Integration Technology

基　　金：深圳市科技计划项目(JCYJ20150401150223635)

摘　　要：骨小梁的生物机制因其复杂的三维微结构和微环境及其与成骨细胞之间的相互作用,已被世界各国学者在骨修复重建和骨再生领域中研究和应用。然而,以往的研究缺乏对体内骨小梁真实环境的模拟,尤其是缺乏对骨小梁微结构的精确量化及其与微环境相互作用条件下骨骼再生的研究与应用。该研究开发一种新的骨小梁仿生微结构的解析与构建方法来分析和构建骨小梁的三维环境。该方法基于Micro CT兔股骨近端松质骨扫描,采用Mimics和Rhino软件构建三维松质骨模型并分析其几何特征,应用斯特林公式和3D打印骨小梁结构,对仿生骨小梁结构进行了解析和构建。截面积对比和结构相似性分析表明,仿生松质骨与天然松质骨结构达到很高的相似度(达95%以上);体外细胞实验表明,仿生骨小梁微环境能够提供较好的细胞生长微环境。该研究可为组织工程临床研究与应用提供有力的工具。The biological mechanism of trabecular bone with its complex three-dimensional microstructure and the interaction between micro-environment and osteoblasts have been studied and applied in the field of bone repair, reconstruction and bone regeneration. However, previous research lack the simulation of the real environment of bone trabecula in vivo, especially the lack of accurate quantification of trabecular microstructure and the research and application of bone regeneration under the condition of interaction with microenvironment. In this study, a new method is used to analyze and construct the three-dimensional environment of trabecular bone. Three-dimensional cancellous bone model and geometric features analysis were conducted by using Mimics and Rhino software based on Micro CT of rabbit proximal femoral cancellous bone scan. Bionic trabecular structure was analyzed and constructed by using Stirling formula and 3 D printed trabecular bone structure. The comparison on trabeculae area and structure similarity analysis between cancellous bone samples and bionic cancellous bone show that bionic cancellous bone has extremely high similarity（more than 95%） with natural cancellous bone structure. In vitro experiments of bionic bone trabecular can construct a good microenvironment for cell growth. In sum, the proposed method provides a useful tool for manufacturing a more realistic physiological structure for clinical research and application for tissue engineering.

关 键 词：骨小梁 解析与构建 三维微结构 仿生 3D打印 

分 类 号：R318.17[医药卫生—生物医学工程]