下丘脑褪黑素受体亚型基因及蛋白表达的研究(英文)  

Melatonin receptor subtype gene and protein expression in human hypothalamus

作　　者：陆祖谦[1] 孙中安[1] 刘志民[1] 何金 刘会敏 彭树勋[3] 

机构地区：[1]第二军医大学长征医院内分泌科,上海200433 [2]长征医院病理科 [3]香港大学医学院生理系

出　　处：《第二军医大学学报》2002年第8期863-867,共5页Academic Journal of Second Military Medical University

基　　金：This work is supported by the HongKong Qiu Cha Foundation(396 6 30 0 8)

摘　　要：目的 :应用 PT- PCR技术和免疫组织化学染色显示人胚胎下丘脑褪黑素受体及其亚型的分布。方法 :取人胚胎下丘脑组织 ,抽提总 RNA并应用 RT- PCR方法检测其 m RNA,扩增所得阳性产物用自动测序仪测序 ;石蜡包埋下丘脑组织 ,应用鼠抗人 m t1 和 MT2 受体单克隆抗体分别检测褪黑素 mt1 和 MT2 受体亚型 m RNA及其蛋白的表达。结果和结论 :人胚胎下丘脑组织中存在 m t1 和 MT2 受体亚型 m RNA的表达及其蛋白的翻译 ,提示在该组织中有 m t1 和 MT2Objective:To identify the melatonin receptor subtype(mt 1 and MT 2) gene and protein distribution in human hypothalamus.Methods:RT PCR technique and immunohistochemistry method were applied to study the distribution of melatonin receptor subtype gene and protein in the hypothalamus of human embryos,respectively.Results:The electrophoresis of RT PCR products with mt 1 and MT 2 subtype revealed specific expression of both melatonin receptor subtypes in human embryonic hypothalamus.Nucleotide sequence analysis indicated that the RT PCR products coincided with cDNA sequence from human GenBank.The mt 1 and MT 2 receptor subtypes with immunohistochemistry method was principally localized to cytoplasm and membrane in the hypothalamus.Conclusion:It is possible that mt 1 and MT 2 subtype of melatonin receptors co exist in the hypothalamus of the human embryos.

关 键 词：下丘脑 褪黑素 mt1受体 基因表达 免疫组织化学 PT-PCR 

分 类 号：Q576[生物学—生物化学] R335.9[医药卫生—人体生理学]