基质金属蛋白酶3在实验性大鼠腹主动脉瘤模型中的表达  

Expression of matrix metalloproteinase-3 in experimental abdominal aortic aneurysm rat model

作　　者：赵新[1] 景在平[1] 熊江[1] 江少杰[1] 

机构地区：[1]第二军医大学长海医院血管外科,全军血管外科研究所上海200433

出　　处：《第二军医大学学报》2002年第8期877-879,共3页Academic Journal of Second Military Medical University

基　　金：军队杰出人才基金资助项目 (98J0 0 5 ) ;上海市卫生系统百名跨世纪优秀学科带头人培养计划基金(97BR0 4 7) ;上海市科技发展基金攻关计划项目(0 0 4 4 190 2 9) ;长海医院学科攀登计划基金资助课题

摘　　要：目的 :检测基质金属蛋白酶 3(MMP- 3)在大鼠正常动脉及腹动脉瘤模型组织中的表达 ,以探讨其在腹主动脉瘤发病机制中的作用。方法 :建立大鼠腹主动脉瘤灌注模型 ,应用免疫组织化学和分子原位杂交技术检测 MMP- 3的表达。结果 :正常动脉壁组织 MMP- 3蛋白表达为弱阳性或阴性 ,而动脉瘤组织中 MMP- 3蛋白表达显著增高 ,阳性率为 8/ 8;MMP- 3阳性染色主要位于中膜的平滑肌细胞和外膜的炎症细胞 ,其中炎症细胞浸润的区域染色明显增强。 MMP- 3m RNA在动脉瘤组织中呈阳性表达 ,在动脉外膜炎症细胞浸润的区域和小血管聚集区呈强阳性 ;对照组动脉组织未见 MMP- 3m RNA阳性表达。 结论 :腹主动脉瘤组织中 MMP- 3蛋白及 m RNA较正常动脉组织表达显著增高 ,尤其在炎症细胞浸润的区域表达明显增强 ;提示 MMP-Objective:To investigate matrix metalloproteinase 3 (MMP 3) expression in experimental abdominal aortic aneurysms (AAA) rat model. Methods:The expression of MMP 3 were observed in 8 rat AAA models and 8 normal control aortic tissues using immunohistochemistry and molecular in situ hybridization technique. Results:The positive rate of MMP 3 protein expression was 8/8 in AAA and it was markedly increased compared with that of control samples( P <0.01). The positive expression of MMP 3 protein, mostly in adventitia inflammatory infiltration areas and media vascular smooth muscle cells in AAA model tissues, was much stronger than that of control aortic tissues. The expression of MMP 3 mRNA in AAA group was much stronger than that in control group. The expression pattern of MMP 3 mRNA in AAA was consistent with expression of MMP 3 protein. Conclusion:The protein and mRNA expression of MMP 3 is markedly increased in experimental AAA than in control abdominal aortic tissues. MMP 3 may be a key initial factor in experimental AAA formation and progression.

关 键 词：基质金属蛋白酶3 大鼠 腹主动脉瘤 免疫组织化学 原位杂交 

分 类 号：R543.16[医药卫生—心血管疾病]