瓜蒌皮提取物对AngⅡ诱导血管平滑肌细胞增殖的影响  

Effect of extractive of pericarpium trichosanthis on the proliferation of rat vascular smooth muscle cell induced by angiotensin Ⅱ

作　　者：杨征[1] 宋淼[2] 陈强 宋宇亭 郭晓华[1] 邱敏[2] 

机构地区：[1]包头医学院第一附属医院心内二科,内蒙古自治区包头市014010 [2]包头医学院药学院,内蒙古自治区包头市014060

出　　处：《临床合理用药杂志》2018年第1期44-46,共3页Chinese Journal of Clinical Rational Drug Use

基　　金：内蒙古自然科学基金(No:2011MS1131;2015MS0809);内蒙古科技计划项目(No:20130414)

摘　　要：目的观察瓜蒌皮提取物对AngⅡ诱导血管平滑肌细胞增殖的影响。方法采用MTS法检测瓜蒌皮提取物(10、20、30 mg·L-1)对血管紧张素Ⅱ(10-7mol·L-1)所致血管平滑肌细胞增殖活性的影响;Real time RTPCR法检测血管平滑肌细胞中内皮型一氧化氮合酶、原癌基因c-fos。结果 MTS检测结果示,与对照组相比,AngⅡ组A490值明显升高(P<0.05),与AngⅡ组相比,AngⅡ+EPT组A490值均明显下降(P<0.05)。Real-time RT-PCR测定结果示,AngⅡ组e NOS mRNA的表达低于对照组,c-fos mRNA的表达高于对照组,差异均有统计学意义(P<0.05);加入EPT后,e NOS mRNA的表达高于AngⅡ组,c-fos mRNA的表达低于AngⅡ组,差异均有统计学意义(P<0.05)。结论瓜蒌皮提取物抑制血管紧张素Ⅱ所致的细胞增殖,其作用机制可能与降低c-fos mRNA高表达,升高一氧化氮合酶mRNA表达有关。Objective To observe the effect of extractive of pericarpium trichosanthis（EPT） on the proliferation of rat vascular smooth muscle cell（VSMC） proliferation induced by antiotensin Ⅱ（Ang Ⅱ） and explore its mechanism. Methods A cell proliferating model of VSMC induced by Ang Ⅱ was established. Effect of EPT（10、20、30 mg·L-1） on Ang Ⅱ（10-7 mol· L-1）-induced VSMC proliferation were evaluated by MTS assay; the mRNA expression of endothelial NO synthase（e NOS） 、c-fos in VSMCs were detected by real-time quantitative reverse transcription-polymerase chain reaction（real-time RTPCR）. Results MTS test showed that： compared with the control group,the A490 value of Ang Ⅱ group increased significantly（P〈0. 05）; compared with Ang Ⅱ group,the A490 value of Ang Ⅱ ＋ EPT group decreased significantly（P〈0. 05）. Real-time RT-PCR analysis showed that： the expression of e NOS mRNA in Ang Ⅱ group was lower than that in the control group,and the expression of c-fos mRNA was higher than that in the control group,the differences were statistically significant（P〈0. 05）. After adding EPT,the expression of e NOS mRNA was higher than that of the group Ang Ⅱ,and the expression of c-fos mRNA was lower than that of the Ang Ⅱ group,and the differences were statistically significant（P〈0. 05）. Conclusion The mechanism of EPT on the anti-proliferation may be related to its down-regulatory effect on c-fos mRNA expression and increased e NOS mRNA expression.

关 键 词：瓜蒌皮提取物 血管紧张素Ⅱ 血管平滑肌细胞 增殖 细胞周期 

分 类 号：R285[医药卫生—中药学]