Cationic conjugated polymers as signal reporter for label-free assay based on targets-mediated aggregation of perylene diimide quencher  

作　　者：Limin Guo Ziqi Zhang Yanli Tang 

机构地区：[1]Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanni Normal University, Xi'an 710062, China

出　　处：《Chinese Chemical Letters》2018年第2期305-308,共4页中国化学快报（英文版）

基　　金：the financial support from the National Natural Science Foundation of China(No. 21675106);the 111 Project(No. B14041);Natural Science Basic Research Plan in Shaanxi Province of China (No. 2017JM2019);the Program for Changjiang Scholars and Innovative Research Team in University (No. 14R33);the Program for Innovative Research Team in Shaanxi Province(No. 2014KCT-28)

摘　　要：Herein, we reported a new label-free and fluorescence turn-on biosensor based on cationic conjugated poly（9,9-bis（6＇-N,N,N-trimethylammonium）hexyl）fluorine phenylene）（PFP） and perylene diimide derivatives（PDI）. Cationic PFP, single-stranded nucleic acid and PDI were used as signal reporter, probe and fluorescence quencher, respectively. In the presence of nucleic acids, they form complexes with PFP and PDI through strong electrostatic attraction interactions, resulting in PDI aggregating on nucleic acids and fluorescence of PFP being quenched. When nucleic acids are hydrolyzed by enzymes or their conformation is changed via recognizing targets, the effective aggregation of PDI is disrupted and the quenching ability is decreased. Thus the fluorescence of PFP recovers significantly. By taking advantage of the mechanism, we construct a new biosensor for endonuclease and small molecules detection. Here, S1 nuclease and bisphenol A are used as model systems. The detection limit of the SI nuclease and BPA are1.0×10^-6U/mL and 0.05 ng/mL, respectively. Our method is sensitive, cost-effective and simple, and provides a new platform for bioanalysis.Herein, we reported a new label-free and fluorescence turn-on biosensor based on cationic conjugated poly（9,9-bis（6＇-N,N,N-trimethylammonium）hexyl）fluorine phenylene）（PFP） and perylene diimide derivatives（PDI）. Cationic PFP, single-stranded nucleic acid and PDI were used as signal reporter, probe and fluorescence quencher, respectively. In the presence of nucleic acids, they form complexes with PFP and PDI through strong electrostatic attraction interactions, resulting in PDI aggregating on nucleic acids and fluorescence of PFP being quenched. When nucleic acids are hydrolyzed by enzymes or their conformation is changed via recognizing targets, the effective aggregation of PDI is disrupted and the quenching ability is decreased. Thus the fluorescence of PFP recovers significantly. By taking advantage of the mechanism, we construct a new biosensor for endonuclease and small molecules detection. Here, S1 nuclease and bisphenol A are used as model systems. The detection limit of the SI nuclease and BPA are1.0×10^-6U/mL and 0.05 ng/mL, respectively. Our method is sensitive, cost-effective and simple, and provides a new platform for bioanalysis.

关 键 词：Conjugated polymers Perylene diimide derivatives Label-free S1 nuclease Bisphenol A Fluorescence turn-on 

分 类 号：O657.3[理学—分析化学]