菘蓝开花抑制基因IiFLC的克隆和表达分析  

Cloning and Expression Analysis of Flowering Inhibitor IiFLC in Isatis indigotica Fort.

作　　者：王莉莉 白钰[1] 张轩毓 刘佳泽 唐晓清[1] 杨杰[2] 王芳权[2] 

机构地区：[1]南京农业大学园艺学院,南京210095 [2]江苏省农业科学院粮食作物研究所,南京210014

出　　处：《分子植物育种》2018年第1期1-8,共8页Molecular Plant Breeding

基　　金：国家自然科学基金(31171486);国家级大学生创新训练项目(201610307032)共同资助

摘　　要：为探索菘蓝生殖生长过程中的开花机理,利用PCR技术对菘蓝FLC基因进行克隆和表达分析。在获得的IiFLC基因的cDNA序列全长中含有一条长为585 bp的开放阅读框,可翻译为编码194个氨基酸残基的多肽链,具有典型的MADS-box结构域。通过与十字花科其他植物中的FLC蛋白质序列的系统进化分析发现,菘蓝与它们具有高度的同源性。应用实时荧光定量PCR技术对山西菘蓝自交一代材料的FLC基因的表达量进行定量分析,结果显示在菘蓝不同的器官中的表达量有显著差异,其中菘蓝根中的基因表达量最高,茎中的表达量最低,并在抽薹现蕾期至果期呈现明显的先减后增的变化趋势。The aim of this study was to explore the flowering mechanism of Isatis indigotica Fort..The FLC gene was cloned and its expression was validated by real-time quantitative PCR technique（qPCR）.The c DNA sequence of IiFLC was obtained,containing a 585 bp open reading frame.It encoded a peptide of 194 amino acid residues which contained a typical MADS-box domain.The phylogenetic analysis showed that it shared high similarity with other plants in Cruciferae.The qPCR results of selfing generation of Isatis indigotica from Shanxi revealed the expression of IiFLC was tissue-specific,in which the expression in root was the highest,whereas the expression in stem was the lowest.In addition,the expression decreased first and then increased obviously in the development periods from bolting to fruit bearing.

关 键 词：菘蓝 FLC基因 组织特异性 表达分析 

分 类 号：Q943.2[生物学—植物学] S567.239[农业科学—中草药栽培]