水稻新基因OsCPSF7启动子克隆及其时空表达特性分析  

Cloning and Spatiotemporal Expression Analysis of OsCPSF7 Gene Promoter in Rice(Oryza sativa L.)

作　　者：张小玲[1,2] 尹显明[1,3] 朱骞 董陈文华[1] 郭效琼 孙明姬 陈丽娟 李东宣[1,2] 

机构地区：[1]云南农业大学稻作研究所,昆明650201 [2]云南农业大学省部共建云南生物资源保护与利用国家重点实验室,昆明650201 [3]楚雄州农业科学研究推广所,楚雄675000

出　　处：《分子植物育种》2018年第1期9-15,共7页Molecular Plant Breeding

基　　金："十三五"国家重点研发计划项目(2016YFD0101101-5);国家自然科学基金(31560115);NSFC-云南联合基金重点项目(U11-36604);省部共建云南生物资源保护与利用国家重点实验室开放课题基金;云南省博士研究生学术新人奖(A3007962)共同资助

摘　　要：CPSF(cleavage and polyadenylation specificity factor),真核细胞mRNA3'端前体加工中起主导作用的蛋白因子。然而迄今对植物和水稻CPSF家族基因及其表达调控元件的克隆和功能的研究还不多。本研究克隆了水稻中一个未知功能基因OsCPSF7的上游2 330 bp启动子区域,构建植物融合表达载体pOsCPSF7:GUS,并获得了转基因水稻植株。组织化学染色结果表明,OsCPSF7基因启动子具有表达活性,可驱动GUS报告基因,在转基因水稻植株不同发育时期的叶枕、叶舌、茎间、小穗及种子柱头基部、胚和胚乳的连接部位均有强烈的表达。结果表明OsCPSF7基因启动子可能参与调控信号转导、逆境应答以及水稻生长和发育。该研究结果为进一步研究水稻OsCPSF7基因启动子的功能及其相关调控机制奠定了基础。The Cleavage and Polyadenylation Specificity Factor（CPSF） is a protein factor that plays a leading role in the 3’ end of eukaryotic precursor m RNA.However,cloning and function studies of CPSF family genes in plants and rice have been quite limited up to now.In this study,the 2 330 bp promoter region of an unknown functional gene in rice OsCPSF7 was cloned to construct plant fusion expression vector pOsCPSF7： GUS and the transgenic rice plants were generated.The results of histochemical staining showed that OsCPSF7 gene promoter had expression activity and could drive GUS reporter gene,which had strong expression at auricle,ligule,node,spikelet,base of stigma,and embryo and endosperm connecting part at different developmental stages of rice.The results indicated that OsCPSF7 gene promoter might be involved in regulating signal transduction,stress responses,and rice growth and development.The results of this study might provide a basis for further study on the functionof OsCPSF7 gene promoter and its relative regulatory mechanisms.

关 键 词：水稻 启动子 OsCPSF7 GUS组织化学分析 表达 

分 类 号：Q943.2[生物学—植物学] S511[农业科学—作物学]