冬枣COBRA基因家族全基因组鉴定及表达分析  

Genome-wide Identification and Expression Analysis of COBRA Gene Family in Ziziphus jujuba

作　　者：盛松柏 田菊 庞晓明[1] 

机构地区：[1]北京林业大学生物科学与技术学院林木育种国家工程实验室林木花卉遗传育种教育部重点实验室,北京100083 [2]内蒙古和盛生态科技研究院有限公司,呼和浩特011517

出　　处：《分子植物育种》2018年第1期61-68,共8页Molecular Plant Breeding

基　　金：科技部"十二五"国家科技支撑计划(2013BAD14B0302);国家自然科学基金项目(31372019);内蒙古自治区科技重大专项(内财教[2014]2020号)共同资助

摘　　要：COBRA作为一类GPI锚定蛋白,主要作用于细胞伸长与纤维素沉积。本研究在"冬枣"全基因组中成功地鉴定到10个冬枣COBRA相似基因(ZjCOBL),并对该基因家族成员进行生物信息学分析,还研究了各成员在‘冬枣’7个不同组织中的表达模式。所有潜在的COBRA相似蛋白都具有CCVS保守结构域,其中8个相似蛋白具有N端信号肽序列,big-PI程序预测出7个蛋白为潜在的GPI锚定蛋白。根据基因结构和系统演化分析等依据,冬枣中的COBRA相似蛋白可以分为两个亚家族(SFs),其中SFⅠ亚家族氨基酸相似性为43.3%到79.9%,SFⅡ亚家族为58.3%到67.9%。10个ZjCOBL基因分别位于枣树12条染色体中的5条染色体和一段scaffold上。Real-time RT-PCR试验表明,所有ZjCOBL基因在冬枣各个器官和不同生长时期均有表达。其中,ZjCOBL7与番茄上的Sl COBRA-like为直系同源基因,具有相似的表达模式,推测ZjCOBL7可能与枣果实的质地和成熟软化相关。COBRA,a kind of GPI anchored protein,is mainly involved in cell expansion and cellulose deposition.We successfully identified 10 COBRA-like genes in‘Dongzao’genome（ZjCOBL）.These genes were analyzed by bioinformatics method and their expression patterns in seven tissues were further investigated.All potential COBRA-like proteins had a conserved CCVS domain,among which eight had N-terminal signal peptide sequence.Seven of the members were predicted as potential GPI anchored proteins using the big-PI program.Based on gene structure and phylogeny analysis,the members could be divided into two subfamilies（SFs）.Among the SFⅠ,the identity ranged from 43.3% to 79.9% while it ranged from 58.3% to 67.9% in the SF Ⅱ.10 ZjCOBL genes we’re located on 5 chromosomes and a scaffold sequence.Real-time RT-PCR showed that all genes expressed in different tissues and developing stages.ZjCOBL7 and SlCOBRA-like are orthologs with similar expression pattern in different tissues,indicating that ZjCOBL7 might play important role in the regulation of fruitdevelopment and ripening in Ziziphus jujuba.

关 键 词：冬枣 COBRA基因家族 系统演化分析 基因表达 

分 类 号：Q943.2[生物学—植物学] S665.1[农业科学—果树学]