寄主和非寄主植物中MMDVd正负链比例的研究  

The Ratio of Positive and Negative Strands of MMDVd in Host and Non-host Plants

作　　者：孔卫青 杨金宏 

机构地区：[1]安康学院陕西省蚕桑重点实验室,安康725000

出　　处：《分子植物育种》2018年第4期1115-1120,共6页Molecular Plant Breeding

基　　金：陕西省青年科技新星项目(No.2013KJXX-96);陕西省教育厅重点实验室科研计划项目(No.13JS003)共同资助

摘　　要：引物特异反转录结合实时荧光定量PCR技术对MMDVd在寄主和非寄主植物体内正负链的积累差异进行了研究。根据MMDVd基因组序列,设计了3对反向互补的反转录引物,对桑树和拟南芥中的MMDVd的正负链进行引物特异的反转录,荧光定量PCR技术对纯化后的c DNA进行定量分析。分析表明,桑树中,3组反转录引物的结果都是负链高于正链,为2~9倍,而拟南芥中,正负链的比例不定,显示了较高的随机性。本研究可为进一步的类病毒的侵染机制及其寄主域的研究提供参考。In this study, the differential accumulation of MMDVd in both host and non-host plants was studied by using specific reverse transcription coupled with real-time quantitative PCR. According to MMDVd genome sequence, 3 pairs of reverse complementary sequences were designed as the reverse transcription specific primers. The specific and negative strand of MMDVd in Morus alba and Arabidopsis thaliana was subjected to primer specific reverse transcription, and the quantitative analysis of purified cDNA was carded out by fluorescent quantitative PCR. The results showed that in Morus alba, the negative was 2-9 times higher than the positive chain. While in Arabidopsis, the ratio of positive and negative chains was variable, indicating higher randomness. This study could provide a reference for further studies on the mechanism of viral infection and its host domain.

关 键 词：桑花叶萎缩类病毒 寄主植物 正负链比例 链特异引物 定量PCR 

分 类 号：Q943.2[生物学—植物学] S888.71[农业科学—特种经济动物饲养]