不同光照条件下玉米苗期叶绿素指数的遗传分析  

Genetic Analysis of Chlorophyll Index in Maize at Seedling Stage under Different Light Intensities

作　　者：王亚辉[1,2] 徐淑兔 郑倩文[1,2] 杨秋涵 何坤辉 聂佳伟 冯永琴 刘建超 薛吉全 

机构地区：[1]西北农林科技大学农学院农业部西北旱区玉米生物学与遗传育种重点试验室,杨凌712100 [2]陕西省玉米工程技术研究中心,杨凌712100

出　　处：《分子植物育种》2018年第4期1236-1243,共8页Molecular Plant Breeding

基　　金：玉米宜机收种质创制与利用项目(2016KTCQ02-05)资助

摘　　要：为了研究玉米苗期叶片叶绿素光照强度响应的遗传规律,本研究以玉米骨干自交系K22与Dan340为父母本构建的192个重组自交系作为研究材料,采用完备区间作图法(ICIM),对苗期玉米不同叶片黑暗处理前后叶绿素指数SPAD进行了QTL定位分析。分析结果共检测到位于1号、2号、5号、6号、8号染色体的19个QTL,单个QTL可解释4.80%~14.32%的表型变异。其中黑暗处理前分别定位到位于1号、2号、5号、6号染色体的10个QTL,黑暗处理后共检测到位于1号、5号和8号染色体的5个QTL;同时以黑暗处理前后的叶绿素指数差值作为考察叶绿素降解的指标,共检测到位于5号、6号和8号染色体的4个QTL。研究结果说明不同部位叶片的叶绿素调控机理有所不同,而且不同部位叶片光照响应也不同,该结果可为探究叶片光照响应遗传机理提供参考。In order to study the inheritance of chlorophyll light intensity response in leaves of maize at seedling stage, 192 recombinant inbred lines constructed with maize backbone inbred line K22 and Dan340 as parents were used as research materials. Inclusive composite interval mapping （ICIM） was used to analyze the QTL location of chlorophyll index SPAD in different leaves before and after dark treatment. Totally, nineteen QTLs located at chromosome 1, 2, 5, 6 and 8 were identified, and a single QTL could explain the phenotypic variation of 4.80%-14.32%. Before dark treatment, ten QTLs located at chromosome 1, 2, 5 and 6 were identified. After dark treatment, 5 QTLs at chromosome 1, 5 and 8 were identified. Furthermore, the chlorophyll index difference before and after dark treatment was used as the index of chlorophyll degradation, and 4 QTL were found on chromosomes 5, 6 and 8. In conclusion, the mechanism of chlorophyll regulation in leaves of different parts might be different, and the light response of leaves in different parts might be different, which could provide a reference for exploring the genetic mechanism of leaf illumination response.

关 键 词：玉米 叶绿素 数量性状位点 光照强度 

分 类 号：S513[农业科学—作物学]