丛粒藻烷类非烃化合物的分离、富集与气相色谱纯化、制备  

Separation,purification,and preparation of botryococcane-related non-hydrocarbons by gas preparative chromatography

作　　者：廖晶[1,2] 张玉娇 卢鸿 冯乔[3] 彭平安[1] 盛国英[1] 

机构地区：[1]中国科学院广州地球化学研究所有机地球化学国家重点实验室,广东广州510640 [2]中国科学院大学,北京100049 [3]山东省沉积成矿作用与沉积矿产重点实验室山东科技大学,山东青岛266000

出　　处：《地球化学》2018年第2期134-140,共7页Geochimica

基　　金：中国科学院战略性先导科技专项A类(XDA14010102);国家重点研发计划"深地资源勘查开采"重点专项(2017YFC060310102);国家自然科学基金(41673066;41473045;41602130;41673045)

摘　　要：气相制备色谱(pGC)作为分离、纯化高纯度单体化合物的有效手段,被广泛应用于许多领域。国外地球化学方面已有众多关于化石燃料生物标志物的应用实例,而国内这方面的应用相对较少。本文选取富含丛粒藻烷类生物标志物的茂名油页岩特殊样品,尝试对非烃馏分中的目标化合物开展分离、富集和纯化、制备。在经过硅胶/氧化铝柱色谱初次分离后,取非烃组分进行纯硅胶柱色谱二次分离,之后再采用气相制备色谱开展精细分离和纯化,成功制备出非烃组分中3个与丛粒藻烷相关联的目标化合物,纯度高达95%以上。以此为例,介绍了气相制备色谱的使用原理和目标化合物分离、富集、纯化的经验。Gas preparative chromatography （pGC） has found extensive application in several fields due to its high efficiency in the preparation of pure compounds; it proved to be effective for fossil fuel biomarker studies, as well. For the special oil shale extracts in the Maoming Basin, which display abundant occurrences of biomarker botryococcanes derived from the green alga Botryococcus braunii, certain target compounds that occurred in the polar fraction had to be separated and purified for structural studies. After primary separation with the conventional ＂SARA＂ method of silica gel/aluminum oxide column chromatography and secondary separation by means of silica gel column chromatography, pGC was used to carry out the final delicate separation and preparation. In this procedure, three non-hydrocarbon target compounds were successfully extracted with high purity （〉95%）. Using this vivid example, the operating principle and procedure of pGC and the separation experience of target compounds are introduced in this paper.

关 键 词：柱色谱 气相制备色谱 生物标志化合物 丛粒藻烷 非烃化合物 

分 类 号：P599[天文地球—地球化学] P593[天文地球—地质学]