新型环介导等温扩增技术开发与应用研究进展  

Recent progress in technology development and application of loop-mediated isothermal amplification

作　　者：张亚平 郑志高[1] 简保磊 朱巍巍 

机构地区：[1]太仓出入境检验检疫局,江苏太仓215400

出　　处：《分子诊断与治疗杂志》2018年第2期138-144,共7页Journal of Molecular Diagnostics and Therapy

基　　金：小鼠转基因及MHV检测技术研究(2017KJ11)

摘　　要：环介导等温扩增技术(loop-mediated isothermal amplification,LAMP)是一种成熟的、应用广泛的检测技术。目前LAMP技术已经经历了十余年的研究和发展,开发了多种新型环介导等温扩增技术。其技术改进主要体现在提高检测特异性、加快反应速度、简化样本处理、实现多重扩增以及应用于现场检测平台等方面。本文从免疫环介导等温扩增、微流体环介导等温扩增、电化学发光环介导等温扩增、分子信标指示环介导等温扩增、多重实时环介导等温扩增、结合FTA卡的环介导等温扩增等新型技术,以及环介导等温扩增技术在简单化、快速化与产物检测特异化等方面的改进进行了综述,为环介导等温扩增技术的进一步发展和实际应用提供依据。Loop-mediated isothermal amplification （LAMP） is an established technology that contin- ues to attract the attention of researchers in many fields. Research and development efforts on LAMP technolo- gy have made much progress, focusing on many different aspects such as improving specificity and reaction speed, simplifying sample processing, realizing multiple amplification, and application on the field platform. In this review, an overview of new LAMP technologies will be provided including immuno-LAMP detection, LAMP methods in microfluidic devices, electrochemiluminescence mediated LAMP, molecular beacon LAMP, multiplex real-time LAMP, FTA-LAMP, and technology improvements in simplification and rapidity and prod- uct detection specificity. The aim of this review is to provide the basis for further LAMP technology develop- ment and practical applications.

关 键 词：环介导等温扩增技术 免疫环介导等温扩增技术 电化学发光 分子信标 

分 类 号：R440[医药卫生—诊断学]