基于人工胃酸水解的黄芪糖指纹图谱的建立及不同种质资源黄芪鉴别  

Establishment of Fingerprint of Astragali Radix Polysaccharides Based on Artificial Acidic Acid Hydrolysis and Identification of Different Germplasm Resources of Astragali Radix

作　　者：李晓霞[1] 王迪 王桂臻[2,3] 刘磊[2,3] 李震宇[2] 秦雪梅[2] 李科[2] 

机构地区：[1]山西省果业工作总站,山西太原030001 [2]山西大学中医药现代研究中心,山西太原030006 [3]山西大学化学化工学院,山西太原030006

出　　处：《中国现代中药》2018年第2期152-158,共7页Modern Chinese Medicine

基　　金：国家自然科学基金资助项目(31200278);教育部博士点新教师类基金项目(20131401120006)

摘　　要：目的:模拟人工胃液条件处理不同种质资源蒙古黄芪(移栽芪和野生芪)和膜荚黄芪(移栽芪和野生芪)细胞可溶性多糖及糖缀合物,获得一系列PACE糖指纹图谱,用于黄芪的种质资源鉴别和评价。方法:利用SMICA软件对数据进行主成分分析及t检验,获得区分不同种质资源黄芪的差异性糖组分。结果:蒙古黄芪中移栽芪和野生芪分离现象明显,且多糖酸解产物中的四糖和六糖可以作为区分生长方式的主要差异性糖片段,野生蒙古黄芪中四糖的含量高于蒙古移栽芪中含量,移栽蒙古黄芪中六糖的含量高于野生芪中含量;三糖和四糖可以作为区分野生蒙古黄芪与野生膜荚黄芪的主要差异性片段;五糖和六糖可以作为区分移栽蒙古黄芪与移栽膜荚黄芪的主要差异性片段。结论:人工胃酸降解的多糖产物能很好的鉴别黄芪种属(野生蒙古黄芪与野生膜荚黄芪、移栽蒙古黄芪与移栽膜荚黄芪),生长方式(移栽蒙古黄芪和野生蒙古黄芪)。本研究为黄芪药材的品质评价奠定了基础。Objective： The simulated artificial acid solution was used to treat soluble polysaccharides and glycoconjugates of Astragalus cells in different germplasm resources of cultivated and wild Astragalus membranaceus var. mongholicus and A. membranaceus,and a series of PACE saccharide fingerprints for the identification and evaluation of the germplasm resources of Astragali Radix. Methods： The data were analyzed by principal component analysis and t-test using SMICA software to obtain the difference between different germplasm resources Astragali Radix. Results： The cultivated A. membranaceus var. mongholicus and wild A. membranaceus var. mongholicus separated significantly,tetrasaccharide and hexasaccharide in the polysaccharides acid hydrolyzate could be used as the main differentially expressed sugar segment to distinguish the growth pattern. The content of the tetrasaccharide in wild A. membranaceus was higher than that A. membranaceus var. mongholicus,and the content of hexose in the cultivated A. membranaceus var. mongholicus washigher than that of the wild A. membranaceus var. mongholicus. Trisaccharide and tetrasaccharide could be used as the main difference between wild A. membranaceus var. mongholicus and wild A. membranaceus. The pentasaccharide and hexasaccharidecould be used as the main differentially expressed fragments to distinguish the cultivated A. membranaceus var. mongholicus and A. membranaceus. Conclusion： Artificial acid-degrading polysaccharide product can well distinguish Astraglaus species and growth mode. Thie study laid the foundation for the quality evaluation of Astragali Radix.

关 键 词：人工胃酸 黄芪 生长方式 多糖 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]