泰山马尾松花粉多糖对ALV-J不同接种途径诱导的鸡群免疫抑制的免疫调理作用  

Immunomodulatory functions of Taishan Pinus massoniana pollen polysaccharide on immunosuppressed chickens infected with ALV-J by different inoculation

作　　者：朱丽君[1] 胡莉萍[2] 董雯雯[1] 马宁宁[1] 周建波[1] 魏凯[1] 王海荣[1] 朱瑞良[1] ZHU Li-jun1, HU Li-ping2, DONG Wen-wen1, MA Ning-ning1, ZHOU Jian-bo1, WEI Kai1, WANG Hai-rong1 ,ZHU Rui-liang1(1. Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention/Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, College of Animal Science and Technology, Shandong Agricultural University, Tai＇ an, Shangdong 271018, China ; 2. Animal Disease Prevention and Control Center of Shandong Province , Jinan 250022,Chin)

机构地区：[1]山东农业大学动物科技学院、山东省动物生物工程与疾病防治重点实验室、山东省畜禽疫病防治工程技术研究中心,山东泰安271018 [2]山东省动物疫病预防与控制中心,山东济南250022

出　　处：《中国兽医学报》2018年第3期549-555,569,共8页Chinese Journal of Veterinary Science

基　　金：山东省高校中医药抗病毒协同创新中心资助项目(XTCX2014B01-08);国家自然科学基金资助项目(31272595);山东省“双一流”奖补资金资助项目(SYL2017YSTD11)

摘　　要：分别经口服、点眼、腹腔注射、肌肉注射模拟水平传播感染J亚群禽白血病病毒（ALV-J）,同时连续10d口服给予泰山松花粉多糖（TPPPS）,分别对各组试验鸡体质量及免疫器官指数、带毒、排毒及抗体、相关免疫学指标进行检测比较。结果显示,饲喂多糖组与接毒组相比,试验鸡体质量及免疫器官指数抑制程度得到改善,血液带毒及泄殖腔排毒量降低,抗体分泌提前、分泌量增加,而IL-2、IL-4细胞因子分泌水平提高,T淋巴细胞转化率、CD4^＋和CD8^＋T细胞数增加,其中以腹腔注射和肌肉注射组TPPPS的免疫调理作用最显著,而健康对照组饲喂TPPPS后试验鸡相关免疫指标水平提高。本研究结果表明,TPPPS对健康雏鸡具有一定的免疫增强作用,口服TPPPS对不同水平传播途径感染ALV-J引起的免疫抑制具有免疫调理作用,TPPPS可以作为免疫增强剂用于防控ALV-J的早期水平感染,降低其免疫抑制程度,减少经济损失。To explore the immunomodulation of Taishan Pinus massoniana pollen polysaccharide （TPPPS） on immunosuppressed chickens infected with subgroup J avian leucosis virus （ALV-J） by different inoculation, one-day-old chickens were inoculated with ALV-J via oral, ophthalmic,intraperitoneal, intramuscular infection similar to horizontal transmission. Simultaneously, orally successive TPPPS administration for 10 days, then the body weight, immune organ index, the dynamic changes of viremia, and viral shedding, antibody secretion and immunological parameters were monitored. The results showed TPPPS delivery can substantially relieve the impaired immunity compared with inoculation group, as characterized by increase in body weight and immune organ index, decline in viremia and viral shedding, antibody secretion in advance and enhance, also increase in lymphocyte transformation rate, CD4 ^＋ and CD8^ ＋ T lymphocytes, cytokines （IL-2, IL-4） secretion. Wherein, TPPPS behaved more significant immunomodulation on intraperitoneal and intramuscular infection group. However, the mock group also received effective changes via TP- PPS. These results indicated that TPPPS had immune enhancement to healthy chickens,it also behaved remarkably immunomodulation on immunosuppressed chickens infected with ALV-J by different inoculation. Thus, TPPPS can be used as an immunoenhancer to reduce the immune suppression and economic losses for ALV-J prevention and control in early infection.

关 键 词：泰山松花粉多糖 J亚群禽白血病病毒 水平传播 免疫抑制 免疫调理 

分 类 号：S852.65[农业科学—基础兽医学]